bs-0657R [Primary Antibody]
MRP1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MRP1

Immunogen Range: 501-600/1531


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 17250

Swiss Prot: O35379

Source: KLH conjugated synthetic peptide derived from mouse MRP1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 170


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Cow
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wen-Qing, Hu, Peng Chun-Wei, and Li Yan. "The expression and significance of P-glycoprotein, lung resistance protein and multidrug resistance-associated protein in gastric cancer." Journal of Experimental & Clinical Cancer Research 28 (2009).Read more>>
  • Yan, Yong‐Rong, et al. "Epithelial‐to‐mesenchymal transition is involved in BCNU resistance in human glioma cells." Neuropathology (2014).Read more>>
  • Peng, Lei, et al. "Development of a novel orthotopic non-small cell lung cancer model and therapeutic benefit of 2’-(2-bromohexadecanoyl)-docetaxel conjugate nanoparticles." Nanomedicine: Nanotechnology, Biology and Medicine (2014).Read more>>
  • Chen et al. Timosaponin A-III reverses multi-drug resistance in human chronic myelogenous leukemia K562/ADM cells via downregulation of MDR1 and MRP1 expression by inhibiting PI3K/Akt signaling pathway. (2016) Int.J.Oncol. 48:2063-70Read more>>
  • Yi et al. Solanine reverses multidrug resistance in human myelogenous leukemia K562/ADM cells by downregulating MRP1 expression. (2018) Oncol.Lett. 15:10070-10076Read more>>
  • Peng Yang. et al. Cucurbitacin E Chemosensitizes Colorectal Cancer Cells via Mitigating TFAP4/Wnt/-Catenin Signaling. J Agr Food Chem. 2020;68(48):14148C1416Read more>>
  • Wei Shunying. et al. Overexpression of circ_CELSR1 facilitates paclitaxel resistance of ovarian cancer by regulating miR-149-5p/SIK2 axis. Anti-Cancer Drug. 2021 Jun;32(5):496-507Read more>>
  • Zipeng Gong. et al. Pharmacokinetic Differences of Wuji Pill Components in Normal and Chronic Visceral Hypersensitivity Irritable Bowel Syndrome Rats Attributable to Changes in Tight Junction and Transporters. FRONT PHARMACOL. 2022; 13: 948678Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat kidney tissue labeled Anti-MRP1 Polyclonal Antibody, Unconjugated (bs-0657R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-MRP1 Polyclonal Antibody, Unconjugated (bs-0657R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Human A549 cell lysates probed with Rabbit Anti-MRP1 Polyclonal Antibody, Unconjugated (bs-0657R) (green) at 1:10 for 30 minutes followed by a FITC conjugated secondary antibody compared to control cells (blue).


K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MRP1 Polyclonal Antibody(bs-0657R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).