bs-0664R-FITC [Conjugated Primary Antibody]
HMGB1 Polyclonal Antibody, FITC Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HMGB1

Immunogen Range: 75-170/215


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3146

Swiss Prot: P09429

Source: KLH conjugated synthetic peptide derived from human HMGB1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles

Background:

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity).

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 25


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhao, Lu, et al. "The high mobility group box 1 protein of< i> Sciaenops ocellatus</i> is a secreted cytokine that stimulates macrophage activation." Developmental & Comparative Immunology 35.10 (2011): 1052-1058.Read more>>
  • Jiang, W., et al. "Curculigoside A attenuates experimental cerebral ischemia injury< i> in vitro</i> and< i> vivo</i>." Neuroscience 192 (2011): 572-579.Read more>>
  • Rui, Zhang, et al. "Omega-3 polyunsaturated fatty acids inhibit the increase in cytokines and chemotactic factors induced in vitro by lymph fluid from an intestinal ischemia-reperfusion injury model." Nutrition (2014).Read more>>
  • Wang, Xin‑Jun, et al. "Clinical and prognostic significance of high-mobility group box-1 in human gliomas." Experimental and Therapeutic Medicine.Read more>>
  • Sun, Shanping, et al. "High mobility group box-1 and its clinical value in breast cancer." OncoTargets and Therapy 8 (2015): 413.Read more>>
  • Li, Yuan-bo, et al. "Methotrexate affects HMGB1 expression in rheumatoid arthritis, and the downregulation of HMGB1 prevents rheumatoid arthritis progression." Molecular and Cellular Biochemistry: 1-10.Read more>>
  • Li, Guofu, et al. "The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats." International Journal of Molecular Medicine 38.3 (2016): 767-775.Read more>>
  • He et al. The effects of n-3 PUFA and intestinal lymph drainage on high-mobility group box 1 and Toll-like receptor 4 mRNA in rats with intestinal ischaemia-reperfusion injury. (2012) Br.J.Nut. 108:883-92Read more>>
  • Wang et al. Clinical and prognostic significance of high-mobility group box-1 in human gliomas. (2015) Exp.Ther.Me. 9:513-518Read more>>
  • Qiao et al. Effect of different 1, 25-(OH)2D3 doses on high mobility group box1 and toll-like receptors 4 expression in lung tissue of asthmatic mice. (2015) Int.J.Clin.Exp.Med. 8:4016-23Read more>>
  • Sun et al. Expression and Significance of High-Mobility Group Protein B1 (HMGB1) and the Receptor for Advanced Glycation End-Product (RAGE) in Knee Osteoarthritis. (2016) Med.Sci.Monit. 22:2105-12Read more>>
  • Hong et al. Luteolin Treatment Protects against Renal Ischemia-Reperfusion Injury in Rats. (2018) Mediators.Inflamm. 2017:9783893Read more>>
  • Xiang et al. Glycyrrhizin suppresses the expressions of HMGB1 and relieves the severity of traumatic pancreatitis in rats. (2014) PLoS.One. 9:e115982Read more>>
  • Shi Q et al. Polychlorinated biphenyl quinone-induced signaling transition from autophagy to apoptosis is regulated by HMGB1 and p53 in human hepatoma HepG2 cells. Toxicol Lett. 2019 May 15;306:25-34. Read more>>
  • Yuan FH et al. microRNA‐30a inhibits the liver cell proliferation and promotes cell apoptosis through the JAK/STAT signaling pathway by targeting SOCS‐1 in rats with sepsis. J Cell Physiol. 2019 Apr 10. Read more>>
  • Diao S et al. IGF2 enhanced the osteo‐/dentinogenic and neurogenic differentiation potentials of stem cells from apical papilla. J Oral Rehabil. 2019 Jul 10. Read more>>
  • Feng B et al. Enhancing Triple Negative Breast Cancer Immunotherapy by ICG‐Templated Self‐Assembly of Paclitaxel Nanoparticles. Advanced Functional Materials,2019 1906605. Read more>>
  • Chen Y et al. Dendritic cells-derived interferon-1 ameliorated inflammatory bone destruction through inhibiting osteoclastogenesis. Cell Death Dis. 2020 Jun 2;11(6):414.Read more>>
  • Hongzhong Cheng. et al. Circular RNA circLPAR3 Facilitates Esophageal Squamous Cell Carcinoma Progression Through Upregulating HMGB1 via Sponging miR-375/miR-433. Oncotargets Ther. 2020; 13: 7759C7771Read more>>
  • Xuting Liu. et al. Amorphous silica nanoparticles induce inflammation via activation of NLRP3 inflammasome and HMGB1/TLR4/MYD88/NF-kb signaling pathway in HUVEC cells. J Hazard Mater. 2021 Feb;404:12405Read more>>
  • Lei Chen. et al. Circ_0032821 Facilitates Gastric Cancer Cell Proliferation, Migration, Invasion and Glycolysis by Regulating MiR-1236-3p/HMGB1 Axis. Cancer Manag Res. 2020; 12: 9965C9976Read more>>
  • Tian Zhang. et al. Supramolecular Tadalafil Nanovaccine for Cancer Immunotherapy by Alleviating Myeloid-Derived Suppressor Cells and Heightening Immunogenicity. 2021 May 13Read more>>
  • Zhen-Han Feng. et al. A combination strategy based on an Au nanorod/doxorubicin gel via mild photothermal therapy combined with antigen-capturing liposomes and anti-PD-L1 agent promote a positive shift in the cancer-immunity cycle. Acta Biomater. 2021 OcRead more>>
  • Yuting Shen. et al. Tailoring Chemoimmunostimulant Bioscaffolds for Inhibiting Tumor Growth and Metastasis after Incomplete Microwave Ablation. Acs Nano. 2021;XXXX(XXX):XXX-XXXRead more>>
VALIDATION IMAGES