bs-0668R [Primary Antibody]
Met (c Met) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Met (c-Met)

Immunogen Range: 25-75/1390

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4233

Swiss Prot: P08581

Source: KLH conjugated synthetic peptide derived from human Met (c-Met)

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months.

Background: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. Acts as a receptor for Listeria internalin inlB, mediating entry of the pathogen into cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gao, Wei, et al. "Study of critical role of c-Met and its inhibitor SU11274 in colorectal carcinoma." Medical Oncology 30.2 (2013): 1-8.Read more>>
  • Yu, Xiuju, et al. "Expression and tissue distribution of hepatocyte growth factor (HGF) and its receptor (c-Met) in alpacas (Vicugna pacos) skins associated with white and brown coat colors." Acta Histochemica (2015).Read more>>
  • Li, Hongyu, et al. "Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer." Oncology letters9.5 (2015): 1989-1996.Read more>>
  • Wang, Xinhong, et al. "Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation." Cytokine (2017).Read more>>
  • Qi et al. Therapeutic role of human hepatocyte growth factor (HGF) in treating hair loss. (2016) Peer. 4:e2624Read more>>
  • Guo et al. Expression of MACC1 and c-Met in human gastric cancer and its clinical significance. (2013) Cancer.Cell.Int. 13:121Read more>>
VALIDATION IMAGES

Mouse nephrocytes probed with Rabbit Anti-Met (c Met) Polyclonal Antibody (bs-0668R) at 6ug for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


Formalin-fixed and paraffin embedded human gastric carcinoma labeled Anti-C-Met Polyclonal Antibody, Unconjugated (bs-0668R) at 1:100, followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-C-Met/Met/HGFR Polyclonal Antibody, Unconjugated(bs-0668R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.