bs-0668R-A350 [Conjugated Primary Antibody]
Met (c Met) Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: Met (c-Met)

Immunogen Range: 25-150/1390


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4233

Swiss Prot: P08581

Source: KLH conjugated synthetic peptide derived from human Met (c-Met)

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. Acts as a receptor for Listeria internalin inlB, mediating entry of the pathogen into cells.

Conjugation: ALEXA FLUOR® 350

Excitation/ Emission: 346nm/442nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 153


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gao, Wei, et al. "Study of critical role of c-Met and its inhibitor SU11274 in colorectal carcinoma." Medical Oncology 30.2 (2013): 1-8.Read more>>
  • Yu, Xiuju, et al. "Expression and tissue distribution of hepatocyte growth factor (HGF) and its receptor (c-Met) in alpacas (Vicugna pacos) skins associated with white and brown coat colors." Acta Histochemica (2015).Read more>>
  • Li, Hongyu, et al. "Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer." Oncology letters9.5 (2015): 1989-1996.Read more>>
  • Wang, Xinhong, et al. "Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation." Cytokine (2017).Read more>>
  • Qi et al. Therapeutic role of human hepatocyte growth factor (HGF) in treating hair loss. (2016) Peer. 4:e2624Read more>>
  • Guo et al. Expression of MACC1 and c-Met in human gastric cancer and its clinical significance. (2013) Cancer.Cell.Int. 13:121Read more>>
  • Shi Yan. et al. The Relationship between MACC1/c-Met/Cyclin D1 Axis Expression and Prognosis in ESCC. Anal Cell Pathol. 2022;2022:9651503Read more>>
  • Tang-Yuan Chu. et al. Effect of ovulation IGF and HGF signaling on the oncogenesis of murine epithelial ovarian cancer cell ID8. EXP CELL RES. 2022 Oct;419:113323Read more>>
  • Tang-Yuan Chu. et al. Insulin-like growth factor (IGF) and hepatocyte growth factor (HGF) in follicular fluid cooperatively promote the oncogenesis of high-grade serous carcinoma from fallopian tube epithelial cells: Dissection of the molecular effects. MOL CARCINOGEN. 2023 JuRead more>>
VALIDATION IMAGES