bs-0670R
[Primary Antibody]
c-Jun Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: c-Jun
Immunogen Range: 31-331/331
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3725
Swiss Prot: P05412
Source: KLH conjugated synthetic peptide derived from human c-Jun
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 43
Cross Reactive Species:
Human
Mouse
Rat
Chicken
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zhang, Jihong, et al. "Interleukin 18 augments growth ability via NF-κB and p38/ATF2 pathways by targeting cylin B1, cyclin B2, cylin A2, and Bcl-2 in BRL-3A rat liver cells." Gene (2015).Read more>>
- Zhou, Zhiwei, et al. "microRNA let-7c is essential for the anisomycin-elicited apoptosis in Jurkat T cells by linking JNK1/2 to AP-1/STAT1/STAT3 signaling." Scientific Reports 6 (2016): 24434.Read more>>
- Zhang, Ying, et al. "Overexpression of WNT5B promotes COLO 205 cell migration and invasion through the JNK signaling pathway." Oncology Reports.Read more>>
- Liu, Lina, et al. "Therapeutic effects of 1, 25-dihydroxyvitamin D3 on diabetes-induced liver complications in a rat model." Experimental and Therapeutic Medicine 11.6 (2016): 2284-2292.Read more>>
- Du, Jinghua, et al. "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." Molecular medicine reports 13.3 (2016): 2159-2166.Read more>>
- Liu et al. Therapeutic effects of 1,25-dihydroxyvitamin D3 on diabetes-induced liver complications in a rat model. (2016) Exp.Ther.Me. 11:2284-2292Read more>>
- Manabe A et al.Upregulation of transient receptor potential melastatin 6 channel expression by rosiglitazone and all-trans-retinoic acid in erlotinib-treated renal tubular epithelial cells.(2018)J Cell Physiol. Read more>>
- Zhu G et al. TRAF6 promotes the progression and growth of colorectal cancer through nuclear shuttle regulation NF-kB/c-jun signaling pathway. Life Sci. 2019 Sep 2;235:116831.Read more>>
- Wu Z et al. Co-infection of Mycoplasma gallisepticum and Escherichia coli Triggers Inflammatory Injury Involving the IL-17 Signaling Pathway. Front Microbiol. 2019 Nov 15;10:2615.Read more>>
- "Zhang C et al. MiR-429 regulates rat liver regeneration and hepatocyte proliferation by targeting JUN/MYC/BCL2/CCND1 signaling pathway.Cell Signal. 2018 Oct;50:80-89."Read more>>
- Mei Ha. et al. PKC mediated by the PI3K/Akt-FOXA1 cascade facilitates cypermethrin-induced hyperthyroidism. Sci Total Environ. 2021 Feb;757:143727Read more>>
- Guangwei Zhu. et al. The Effects of TRAF6 on Growth and Progression in Colorectal Cancer are Regulated by miRNA-140. Oncotargets Ther. 2020; 13: 11991C12001Read more>>
- Jinhao Zeng. et al. Ginsenoside Rb1 Lessens Gastric Precancerous Lesions by Interfering With -Catenin/TCF4 Interaction. Front Pharmacol. 2021; 12: 682713Read more>>
- Que, Tianshi. et al. HMGA1 stimulates MYH9-dependent ubiquitination of GSK-3 via PI3K/Akt/c-Jun signaling to promote malignant progression and chemoresistance in gliomas. Cell Death Dis. 2021 Dec;12(12):1-12Read more>>
- Zhao, Lifang. et al. Real-world PM2.5 exposure induces pathological injury and DNA damage associated with miRNAs and DNA methylation alteration in rat lungs. Environ Sci Pollut R. 2022 Jan;:1-16Read more>>
- Cuifang Chang. et al. The orphan GPR50 receptor interacting with T_RI induces G1/S-phase cell cycle arrest via Smad3-p27/p21 in BRL-3A cells. BIOCHEM PHARMACOL. 2022 Aug;202:115117Read more>>
- Xiaobao Gong. et al. Synthesis and anti-inflammatory activity of paeonol derivatives with etherized aryl urea by regulating TLR4/MyD88 signaling pathway in RAW264.7 cell. BIOORG CHEM. 2022 Oct;127:105939Read more>>
- Chunyan Zhang. et al. Overexpression of miR-125a-5p Inhibits Hepatocyte Proliferation through the STAT3 Regulation In Vivo and In Vitro. INT J MOL SCI. 2022 Jan;23(15):8661Read more>>
- Jun Liu. et al. Pharmacology Mechanism of <i>Polygonum Bistorta</i> in Treating Ulcerative Colitis Based on Network Pharmacology. EVID-BASED COMPL ALT. 2022 Dec 28;2022:646156Read more>>
- Xiaoxiang Peng. et al. Phasic regulation of the ATP/P2X7 receptor signaling pathway affects the function of antigen-presenting cells in experimental autoimmune uveitis. INT IMMUNOPHARMACOL. 2023 Jun;119:110241Read more>>
- Hao Huang. et al. MAP4K4 is involved in the neuronal development of retinal photoreceptors. EXP EYE RES. 2023 Aug;233:109524Read more>>
- Sun Jia. et al. ANKRD49 promotes the metastasis of NSCLC via activating JNK-ATF2/c-Jun-MMP-2/9 axis. BMC CANCER. 2023 Dec;23(1):1-18Read more>>
VALIDATION IMAGES
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-c-Jun Polyclonal Antibody (bs-0670R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.\n
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-c-Jun Polyclonal Antibody (bs-0670R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-C-Jun Polyclonal Antibody (bs-0670R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with c-Jun Polyclonal Antibody, Unconjugated (bs-0670R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with c-jun Polyclonal Antibody, Unconjugated (bs-0670R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃ and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with C-jun Polyclonal Antibody(bs-0670R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HepG2 cells probed with c-Jun Polyclonal Antibody, unconjugated (bs-0670R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
Lane 1: Mouse Kidney lysates; Lane 2: Mouse Lung lysates; Lane 3: Mouse NIH/3T3 cell lysates; Lane 4: Human Hela cell lysates probed with c-Jun Polyclonal Antibody, Unconjugated (bs-0670R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.