bs-0678R-FITC [Conjugated Primary Antibody]
Dnmt1 Polyclonal Antibody, FITC Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Dnmt1

Specificity: This antibody will not recongize isoform 3.

Immunogen Range: 21-120/1616


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1786

Swiss Prot: P26358

Source: KLH conjugated synthetic peptide derived from human Dnmt1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 178


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Khan, Md Asaduzzaman, et al. "Thymoquinone inhibits cancer metastasis by downregulating TWIST1 expression to reduce epithelial to mesenchymal transition." Oncotarget.Read more>>
  • Gökmen, Esra, and Kemal Ergin. "DNA Methyltransferase Expression and Proliferation Status of Metastatic Breast Cancer Cell Line After Prolonged and Repeated Rapamycin and Melatonin Application." Meandros Medical Journal.Read more>>
  • Huang et al. Expression of DNA methyltransferases and target microRNAs in human tissue samples related to sporadic colorectal cancer. (2016) Oncol.Rep. 36:2705-2714Read more>>
  • Chen X et al. Clinical and Histological Features of Intraoral Flap and a Preliminary Study of DNA Methylation of Mucosalization. Indian Journal of Surgery. 2020.Read more>>
  • Xiao-Tan Zhang. et al. Baicalin reversal of DNA hypermethylation-associated Klotho suppression ameliorates renal injury in type 1 diabetic mouse model. Cell Cycle. 2020;19(23):3329-3347Read more>>
  • Ma, Wenbin. et al. CircPCNX Promotes PDGF-BB-Induced Proliferation and Migration of Human Aortic Vascular Smooth Muscle Cells Through Regulating miR-1278/DNMT1 Axis. CARDIOVASC DRUG THER. 2022 Jun;:1-13Read more>>
  • Ruimeng Liu. et al. Protective role of curcumin on broiler liver by modulating aflatoxin B1-induced DNA methylation and CYPs expression. ECOTOX ENVIRON SAFE. 2023 Jul;260:115086Read more>>