bs-0685R [Primary Antibody]
SYK Polyclonal Antibody
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Host: Rabbit

Target Protein: SYK

Immunogen Range: 501-600/635


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6850

Source: KLH conjugated synthetic peptide derived from human SYK

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Syk (72 kDa) is a non receptor protein tyrosine kinase that plays an important role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration. SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions. Alternate Names: p72syk; Spleen tyrosine kinase; Tyrosine protein kinase SYK.

Size: 100uL, 20uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 72


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Fu S et al. Berberine suppresses mast cell-mediated allergic responses via regulating FcɛRI-mediated and MAPK signaling.Int Immunopharmacol. 2019 Jun;71:1-6. Read more>>
  • Xin Shan. et al. Proteomic analysis of healthy and atretic porcine follicular granulosa cells. J Proteomics. 2021 Feb;232:104027Read more>>
  • Gao, Jia Rong. et al. MicroRNA-339-5p inhibits lipopolysaccharide-induced rat mesangial cells by regulating the Syk/Ras/c-Fos pathway. N-S ARCH PHARMACOL. 2022 Jun;:1-11Read more>>
  • Xiangyu Liang. et al. Effects and potential mechanisms of Saposhnikovia divaricata (Turcz.) Schischk. On type I allergy and pseudoallergic reactions in vitro and in vivo. J ETHNOPHARMACOL. 2024 Jan;318:116942Read more>>
  • Xiao-Rong Huang. et al. Macrophage autophagy protects against acute kidney injury by inhibiting renal inflammation through the degradation of TARM1. AUTOPHAGY. 2024 29Read more>>
VALIDATION IMAGES

Mouse Spleen lysates probed with SYK Polyclonal Antibody, Unconjugated (bs-0685R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


The Raji (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) .Cells stained with Primary Antibody for 30 min at room temperature, followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-SYK antibody (bs-0685R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.