bs-0698R [Primary Antibody]
IL-10 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IL-10

Immunogen Range: 101-178/178

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 16153

Swiss Prot: P18893

Source: KLH conjugated synthetic peptide derived from mouse IL-10

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background:

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 18

Cross Reactive Species: Human
Mouse
Rat
Rabbit
Others

Predicted Cross Reactive Species: Sheep
Pig
Chicken
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Kou, Wei, et al. "Transforming growth factor-β1 promotes Treg commitment in nasal polyposis after intranasal steroid treatment." Inflammation Research (2013): 1-7.Read more>>
  • Luo, Kai, et al. "Association between Postmenopausal Osteoporosis and Experimental Periodontitis." BioMed Research International 2014 (2014).Read more>>
  • Castoldi, Giovanna, et al. "PREVENTION OF DIABETIC NEPHROPATHY BY COMPOUND 21, SELECTIVE AGONIST OF THE ANGIOTENSIN TYPE 2 RECEPTORS, IN ZUCKER DIABETIC FATTY RATS." American Journal of Physiology Renal Physiology (2014): ajprenal-00247.Read more>>
  • Rohmah, Rista Nikmatu, Edi Widjajanto, and Fatchiyah Fatchiyah. "Protective effect of CSN1S2 protein of goat milk on ileum microstructure and inflammation in rat-CFA-induced rheumatoid arthritis." Asian Pacific Journal of Tropical Disease 5.7 (2015): 564-568.Read more>>
  • Arendt, Maria K., et al. "Interleukin-10 neutralizing antibody for detection of intestinal luminal levels and as a dietary additive in Eimeria challenged broiler chicks." Poultry Science (2016): pev365.Read more>>
  • Pylayeva-Gupta, Yuliya, et al. "IL-35 producing B cells promote the development of pancreatic neoplasia." Cancer discovery (2015): CD-15.Read more>>
  • Han, Sheng, et al. "LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4." Journal of Experimental & Clinical Cancer Research 36.1 (2017): 83.Read more>>
  • Luo et al. Association between postmenopausal osteoporosis and experimental periodontitis. (2014) Biomed.Res.In. 2014:316134Read more>>
  • Chen et al. Hydrogen sulfide reduces kidney injury due to urinary-derived sepsis by inhibiting NF-κB expression, decreasing TNF-α levels and increasing IL-10 levels. (2014) Exp.Ther.Me. 8:464-470Read more>>
  • Kemmler et al. Exposure to 100% Oxygen Abolishes the Impairment of Fracture Healing after Thoracic Trauma. (2015) PLoS.On. 10:e0131194Read more>>
  • Corradetti et al. Immune tuning scaffold for the local induction of a pro-regenerative environment. (2017) Sci.Rep. 7:17030Read more>>
  • Wu Y et al. Tetramethylpyrazine (TMP) ameliorates corneal neovascularization via regulating cell infiltration into cornea after alkali burn. (2019)Biomedicine & Pharmacotherapy, 109, 1041–1051. Read more>>
  • Wang Y et al. Aspirin inhibits inflammation and scar formation in the injury tendon healing through regulating JNK/STAT‐3 signalling pathway. Cell Prolif. 2019 Jun 21:e12650. Read more>>
  • Liu Y et al. Calycosin-7-O-β-D-glucoside attenuates myocardial ischemia-reperfusion injury by activating JAK2/STAT3 signaling pathway via the regulation of IL-10 secretion in mice. Mol Cell Biochem. 2019 Nov 11.Read more>>
  • Yamada H et al. LATE-ONSET ALZHEIMER'S DISEASE ANIMAL MODEL AND USES THEREOF. US Patent20190387723Read more>>
  • Jin Z et al. Geniposide alleviates atherosclerosis by regulating macrophage polarization via the FOS/MAPK signaling pathway. Biomed Pharmacother. 2020 May;125:110015. Read more>>
  • Hou X et al. A 2D-2D heterojunction Bi2WO6/WS2-x as a broad-spectrum bactericide: Sulfur vacancies mediate the interface interactions between biology and nanomaterials. Biomaterials. 2020 Jun;243:119937. Read more>>
  • Liang Wang et al. Clinical Significance of M1/M2 Macrophages and Related Cytokines in Patients with Spinal Tuberculosis. Dis Markers. 2020 May 20;2020:2509454.Read more>>
  • Zhang Q et al. Transcriptome Analysis of the Gene Expression Profiles Associated with Fungal Keratitis in Mice Based on RNA-Seq. Invest Ophthalmol Vis Sci. 2020 Jun 3;61(6):32.Read more>>
  • Yitian Du. et al. Engineered Microglia Potentiate the Action of Drugs against Glioma Through Extracellular Vesicles and Tunneling Nanotubes. 2021 Feb 28Read more>>
  • Xiaoli Gao. et al. Epigenetic upregulation of ssc-miR-124a following treatment with Clostridium perfringens beta2-toxin attenuates both apoptosis and inflammation in intestinal porcine epithelial cells. Arch Biochem Biophys. 2021 Apr;701:108806Read more>>
  • Yongmei Liu. et al. Fecal transplantation alleviates acute liver injury in mice through regulating Treg/Th17 cytokines balance. Sci Rep-Uk. 2021 Jan;11(1):1-1Read more>>
  • Lushuang Xie. et al. Inflammatory factors and amyloid -induced microglial polarization promote inflammatory crosstalk with astrocytes.. Aging-Us. 2020 Nov 30; 12(22): 22538C22549Read more>>
  • Chao Meng. et al. IKK deficiency inhibits acute lung injury following renal ischemia reperfusion injury. Mol Med Rep. 2020 Nov;22(5):4213-422Read more>>
  • Chinthalapally V. Rao. et al. GSK3\ARC/Arg3.1 and GSK3\Wnt signaling axes trigger amyloid\ accumulation and neuroinflammation in middle\aged Shugoshin 1 mice. Aging Cell. 2020 Oct;19(10):e13221Read more>>
  • Shanshan Zhao. et al. IL\27 R+ cells promoted allorejection via enhancing STAT1/3/5 phosphorylation. J Cell Mol Med. 2020 Sep;24(18):10756-10767Read more>>
  • Jia-xiang Zhang. et al. Kupffer cell depletion attenuates IL-6/STAT3 mediates hepatocyte apoptosis in immunological liver injury of trichloroethylene sensitized mice. Int Immunopharmacol. 2020 Nov;88:106897Read more>>
  • Darius H?usler. et al. CNS inflammation after natalizumab therapy for multiple sclerosis: A retrospective histopathological and CSF cohort study. 2021 May 06Read more>>
  • Yan Zhang. et al. Polysaccharide from Ganoderma lucidum ameliorates cognitive impairment by regulating the inflammation of the brainCliver axis in rats. 2021 May 18Read more>>
  • Yanhong Li. et al. Therapeutic potential of human umbilical cord mesenchymal stem cells on aortic atherosclerotic plaque in a high-fat diet rabbit model. Stem Cell Res Ther. 2021 Dec;12(1):1-14Read more>>
  • Xueli Bai. et al. Regulatory role of methionine enkephalin in myeloid-derived suppressor cells and macrophages in human cutaneous squamous cell carcinoma. Int Immunopharmacol. 2021 Oct;99:107996Read more>>
  • Fangyu Qiao. et al. 4-Octyl itaconate modified demineralized bone matrix scaffold improves bone repair by regulating early inflammation. Chem Eng J. 2021 Dec;425:13149Read more>>
  • Dongdong Yao. et al. Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation. Mat Sci Eng C-Mater. 2021 Sep;128:112359Read more>>
  • Si-Cheng Zhao. et al. Nickel sulfate exposure induces ovarian inflammation and fibrosis and decreases oocyte quality in mice. Ecotox Environ Safe. 2021 Nov;224:112634Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colon tissue labeled Anti-IL-10 Polyclonal Antibody, Unconjugated(bs-0698R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-0698R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Mouse spleen lysates probed with IL-10 Polyclonal Antibody, Unconjugated (bs-0698R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human THP-1 cell lysates; Lane 2: Human THP-1-LPS cell lysates probed with IL-10 Polyclonal Antibody, Unconjugated (bs-0698R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.