Host: Rabbit
Target Protein: LDL receptor
Immunogen Range: 781-860/860
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3949
Swiss Prot: P01130
Source: KLH conjugated synthetic peptide derived from human LDL-R
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 92
Cross Reactive Species:
Human
Mouse
Rat
Plant
Hamster
(Hamster)
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Wu, Hao, et al. "The antihypercholesterolemic effect of jatrorrhizine isolated from Rhizoma Coptidis." Phytomedicine (2014).Read more>>
- Kou, Shuming, et al. "Synergetic cholesterol-lowering effects of main alkaloids from Rhizoma Coptidis in HepG2 cells and hypercholesterolemia hamsters." Life Sciences (2016).Read more>>
- Jia, Lianqun., et al. "Effects of Tanshinone IIA on the modulation of miR‑33a and the SREBP‑2/Pcsk9 signaling pathway in hyperlipidemic rats." Molecular Medicine Reports (2016).Read more>>
- Saxena et al. Differential Response of B Cells to an Immunogen, a Mitogen and a Chemical Carcinogen in a Mouse Model System. (2018) Asian.Pac.J.Cancer.Prev. 19:81-90Read more>>
- Grube,et al.Mining the Secretome of C2C12 Muscle Cells: Data Dependent Experimental Approach To Analyze Protein Secretion Using Label-Free Quantification and Peptide Based Analysis.(2018) Journal of Proteome Research. 17:879-890.Read more>>
- Kwon,et al.Luterion and separating and culturing methods for same.(2017) . Read more>>
- Kaur AP et al. Differential response of T cells to an immunogen, a mitogen and a chemical carcinogen in a mouse model system. J Biochem Mol Toxicol. 2019 May;33(5):e22290. Read more>>
- Kyoung Jin Leeet al. Real-time monitoring of oncolytic VSV properties in a novel in vitro microphysiological system containing 3D multicellular tumor spheroids. PLoS One. 2020 Jul 6;15(7):e0235356.Read more>>
- Wei Zou. et al. Imperatae rhizoma-Hedyotis diffusa Willd. herbal pair alleviates nephrotic syndrome by integrating anti-inflammatory and hypolipidaemic effects. Phytomedicine. 2021 Sep;90:153644Read more>>
- Mei Hui. et al. The Hypolipidemic Effect of Dalbergia odorifera T. C. Chen Leaf Extract on Hyperlipidemic Rats and Its Mechanism Investigation Based on Network Pharmacology. Evid-Based Compl Alt. 2021;2021:3155266Read more>>
- Huiming Hu. et al. The Hypolipidemic Effect of Hawthorn Leaf Flavonoids through Modulating Lipid Metabolism and Gut Microbiota in Hyperlipidemic Rats.. EVID-BASED COMPL ALT. 2022 Nov;2022:3033311-3033311Read more>>
- Shuzhou Wang. et al. Effects of NAC assisted insulin on cholesterol metabolism disorders in canine type 1 diabetes mellitus. LIFE SCI. 2022 Dec;:121193Read more>>
Human colon lysates probed with Anti LDL Receptor Polyclonal Antibody, Unconjugated (bs-0705R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT.
HepG2 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with LDL receptor Polyclonal Antibody(bs-0705R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: K562 cell lysates; Lane 2: Raw264.7 cell lysates; Lane 3: HepG2 cell lysates; Lane 4: SW480 cell lysates probed with LDL receptor Polyclonal Antibody, Unconjugated (bs-0705R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.