bs-0708R
[Primary Antibody]
Neurofilament H Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Neurofilament H
Immunogen Range: 1-100/1026
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4744
Swiss Prot: P12036
Source: KLH conjugated synthetic peptide derived from human Neurofilament H
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 118
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Cheng, Lei, et al. "Lentiviral-mediated transfer of CDNF promotes nerve regeneration and functional recovery after sciatic nerve injury in adult rats."Biochemical and Biophysical Research Communications?(2013).Read more>>
- Wang, Nan, et al. "Myocardin-related transcription factor-A is key regulator in retinoic acid-induced neural-like differentiation of adult bone marrow-derived mesenchymal stem cells." Gene (2013).Read more>>
- Zhao, Hua, et al. "Transplantation of Cerebral Dopamine Neurotrophic Factor Transducted BMSCs in Contusion Spinal Cord Injury of Rats: Promotion of Nerve Regeneration by Alleviating Neuroinflammation." Molecular neurobiology (2014): 1-13.Read more>>
- Chen S et al. PAM/GO/Gel/SA composite hydrogel conduit with bioactivity for repairing peripheral nerve injury. J Biomed Mater Res A. 2019 Jan 31.Read more>>
- Qiang Denget al. The role of protein kinase C alpha in tri\ortho\cresyl phosphate\induced autophagy in Human neuroblastoma SK\N\SH cells. J Appl Toxicol. 2020 Nov;40(11):1480-1490.Read more>>
- Gang Wang. et al. MiR-195-5p inhibits proliferation and invasion of nerve cells in Hirschsprung disease by targeting GFRA4. 2021 Jan 3Read more>>
- Laura E. Bruijn. et al. Extreme Diversity of the Human Vascular Mesenchymal Cell Landscape. J Am Heart Assoc. 2020;9:e017094Read more>>
- Jian Kang. et al. Matrine treatment reduces retinal ganglion cell apoptosis in experimental optic neuritis. Sci Rep-Uk. 2021 May;11(1):1-12Read more>>
- Erdal Ayhan I_ik. et al. Use of Erythropoietin and Fibrin Glue Mixture for Peripheral Nerve Repair. Plast Reconstr Surg. 2022 Feb;149(2):395-403Read more>>
- Y?lmaz, Mahmut Muhsin. et al. Biological nerve conduit model with de-epithelialized human amniotic membrane and adipose-derived mesenchymal stem cell sheet for repair of peripheral nerve defects. CELL TISSUE RES. 2022 Dec;:1-18Read more>>
- orgonetti, Vittoria. et al. Posttranscriptional Regulation of Gene Expression Participates in the Myelin Restoration in Mouse Models of Multiple Sclerosis: Antisense Modulation of HuR and HuD ELAV RNA Binding Protein. MOL NEUROBIOL. 2023 Jan;:1-17Read more>>
- Yu Chen. et al. Effect of necrostatin-1 on sciatic nerve crush injury in rat models. J ORTHOP SURG RES. 2023 Dec;18(1):1-9Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded mouse brain tissue labeled with Rabbit Anti-NF-H/Neurofilament H/Neurofilament 200 Polyclonal Antibody (bs-0708R), Unconjugated followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat spinal cord labeled with Rabbit Anti-NF-H/Neurofilament H/Neurofilament 200 Polyclonal Antibody (bs-0708R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-NF-H/Neurofilament H/Neurofilament 200 Polyclonal Antibody (bs-0708R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NF-H) Polyclonal Antibody, Unconjugated (bs-0708R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NF-H) Polyclonal Antibody, Unconjugated (bs-0708R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NF-H) Polyclonal Antibody, Unconjugated (bs-0708R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.