bs-0709R [Primary Antibody]
Collagen 2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Collagen 2

Immunogen Range: 1201-1300/1487


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1280

Swiss Prot: P02458

Source: KLH conjugated synthetic peptide derived from human Collagen II

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 117


Cross Reactive Species: Human
Mouse
Rat
Rabbit
Guinea Pig

Predicted Cross Reactive Species: Dog
Cow
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Yang, et al. "Evaluation of three-dimensional scaffolds prepared from poly (3-hydroxybutyrate-< i> co</i>-3-hydroxyhexanoate) for growth of allogeneic chondrocytes for cartilage repair in rabbits." Biomaterials 29.19 (2008): 2858-2868.Read more>>
  • Luo, Yang, et al. "Alendronate Retards the Progression of Lumbar Intervertebral Disc Degeneration in Ovariectomized Rats." Bone (2013).Read more>>
  • Li, Guo, et al. "Poly (3-hydroxybutyrate-co-4-hydroxybutyrate) Based Electrospun 3D Scaffolds for Delivery of Autogeneic Chondrocytes and Adipose-Derived Stem Cells: Evaluation of Cartilage Defects in Rabbit." Journal of Biomedical Nanotechnology 11.1 (2015): 105-116.Read more>>
  • Kuo, Chang-Yi, et al. "Incorporation of chitosan in biomimetic gelatin/chondroitin-6-sulfate/hyaluronan cryogel for cartilage tissue engineering." Carbohydrate Polymers (2014).Read more>>
  • Fu, Na, et al. "P34HB film promotes cell adhesion, in vitro proliferation, and in vivo cartilage repair." RSC Advances (2015).Read more>>
  • Chen, Ying-Chen, et al. "Development and characterization of acellular extracellular matrix scaffolds from porcine menisci for use in cartilage tissue engineering." Tissue Engineering ja (2015).Read more>>
  • Liu, Yong, et al. "Lentivirus-mediated TGF-β3, CTGF and TIMP1 gene transduction as a gene therapy for intervertebral disc degeneration in an in vivo rabbit model." Experimental and Therapeutic Medicine.Read more>>
  • Sheu, Ming-thau, and Ying-chen Chen. "METHOD FOR PRODUCTION OF DECELLULARIZED BIOLOGICAL MATERIAL AND THE DECELLULARIZED BIOLOGICAL MATERIAL PREPARED THEREFROM." U.S. Patent No. 20,150,344,842. 3 Dec. 2015.Read more>>
  • Wang, Chen-Chie, et al. "Expandable Scaffold Improves Integration of Tissue Engineered Cartilage: An In Vivo Study in A Rabbit Model." Tissue Engineering ja (2016).Read more>>
  • Zheng, Li, et al. "Comparative study of collagen hydrogels modified in two ways using the model of ectopic cartilage construction with diffusion-chamber in immunocompetent host." Journal of applied biomaterials & functional materials 12.1 (2014).Read more>>
  • Fu, Na, et al. "PCL‐PEG‐PCL film promotes cartilage regeneration in vivo."Cell Proliferation (2016).Read more>>
  • Li et al. Vitamin D prevents articular cartilage erosion by regulating collagen II turnover through TGF-β1 in ovariectomized rats. (2016) Osteoarthritis.Cartilag. 24:345-53Read more>>
  • Jiang et al. Effect of calcitonin pretreatment on naturally occurring intervertebral disc degeneration in guinea pig. (2015) Int.J.Clin.Exp.Me. 8:10367-79Read more>>
  • Jeong et al. Serial changes in the proliferation and differentiation of adipose-derived stem cells after ionizing radiation. (2016) Stem.Cell.Res.The. 7:117Read more>>
  • Xu et al. Sanmiao formula inhibits chondrocyte apoptosis and cartilage matrix degradation in a rat model of osteoarthritis. (2014) Exp.Ther.Me. 8:1065-1074Read more>>
  • Wu et al. Subchondral bone changes and chondrogenic capacity of progenitor cells from subchondral bone in the collagenase-induced temporomandibular joints osteoarthritis rabbit model. (2015) Int.J.Clin.Exp.Patho. 8:9782-9Read more>>
  • Chen et al. Dual Function of Glucosamine in Gelatin/Hyaluronic Acid Cryogel to Modulate Scaffold Mechanical Properties and to Maintain Chondrogenic Phenotype for Cartilage Tissue Engineering. (2016) Int.J.Mol.Sci. 17 Read more>>
  • Chen et al. Effect of Cyclic Dynamic Compressive Loading on Chondrocytes and Adipose-Derived Stem Cells Co-Cultured in Highly Elastic Cryogel Scaffolds. (2018) Int.J.Mol.Sci. 19 Read more>>
  • Chen et al. Effect of Cyclic Dynamic Compressive Loading on Chondrocytes and Adipose-Derived Stem Cells Co-Cultured in Highly Elastic Cryogel Scaffolds. (2018) Int.J.Mol.Sci. 19 Read more>>
  • Chen XY et al. Pulsed Magnetic Field Stimuli Can Promote Chondrogenic Differentiation of Superparamagnetic Iron Oxide Nanoparticles-Labeled Mesenchymal Stem Cells in Rats.(2018) J Biomed Nanotechnol. 14(12):2135-2145. Read more>>
  • Zhou H et al.Leukemia inhibitory factor promotes extracellular matrix synthesis in degenerative nucleus pulposus cells via MAPK-ERK1/2 signaling pathway.(2018)Biochem Biophys Res Commun;507(1-4):253-2Read more>>
  • Zhao R et al. Interleukin-1 receptor antagonist protein (IL-1Ra) and miR-140 overexpression via pNNS-conjugated chitosan-mediated gene transfer enhances the repair of full-thickness cartilage defects in a rabbit model. Bone Joint Res. 2019 Apr 2;8(3):165-178.Read more>>
  • Han Y et al. Storage solution containing hydrogen improves the preservation effect of osteochondral allograft.Cell Tissue Bank. 2019 Feb 26.Read more>>
  • Wang D et al. Effects of hypoxia and ASIC3 on nucleus pulposus cells: From cell behavior to molecularmechanism. Biomed Pharmacother. 2019 Jun 12;117:109061. Read more>>
  • Zhang J et al. Icariin-conditioned serum engineered with hyaluronic acid promote repair of articular cartilage defects in rabbit knees. BMC Complement Altern Med. 2019 Jul 3;19(1):155. Read more>>
  • Wang HC et al. Intra-articular injection of N-acetylglucosamine and hyaluronic acid combined with PLGA scaffolds for osteochondral repair in rabbits. PLoS One. 2018 Dec 31;13(12):e0209747. Read more>>
  • Sasono B et al. The Effect of Estrogen on Type 2 Collagen Levels in the Joint Cartilage of Post-Menopausal Murine Subjects. Journal of Hard Tissue Biology.2019 28(3):245-250Read more>>
  • Lei J et al. LncRNA SNHG1 Alleviates IL-1β-induced Osteoarthritis by Inhibiting miR-16-5p-mediated p38 MAPK and NF-κB Signaling Pathways. Biosci Rep. 2019 Aug 5. pii: BSR20191523.Read more>>
  • Zhan X et al. Effect of matrix stiffness and adhesion ligand density on chondrogenic differentiation of mesenchymal stem cells. J Biomed Mater Res A. 2019 Nov 20. Read more>>
  • Yang KC et al. Effects of scaffold geometry on chondrogenic differentiation of adipose-derived stem cells. Materials Science and Engineering: C, 110733.Read more>>
  • Yang J et al. Establishment and characterization of an immortalized human chondrocyte cell line. Biotechnol Lett. 2020 Feb 10.Read more>>
  • Xiaoxia Haoet al. Effects of body weight\supported treadmill training on cartilage\subchondral bone unit in the rat model of post\traumatic osteoarthritis. J Orthop Res. 2020 Jun 30.Read more>>
  • Carolina C. Zuliani. et al. Chondrogenesis of human amniotic fluid stem cells in Chitosan-Xanthan scaffold for cartilage tissue engineering. Sci Rep-Uk. 2021 Feb;11(1):1-9Read more>>
  • Rui Zhang. et al. Allogeneic adipose-derived mesenchymal stem cells promote the expression of chondrocyte redifferentiation markers and retard the progression of knee osteoarthritis in rabbits. Am J Transl Res. 2021; 13(2): 632C645Read more>>
  • Ke Zhang. et al. Silencing of Vangl2 attenuates the inflammation promoted by Wnt5a via MAPK and NF-B pathway in chondrocytes. J Orthop Surg Res. 2021 Dec;16(1):1-1Read more>>
  • Chen-Chie Wang. et al. Infrapatellar Fat PadsCDerived Stem Cell Is a Favorable Cell Source for Articular Cartilage Tissue Engineering: An In Vitro and Ex Vivo Study Based on 3D Organized Self-Assembled Biomimetic Scaffold:. Cartilage. 0;()Read more>>
  • Jihang Dai. et al. Glabridin inhibits osteoarthritis development by protecting chondrocytes against oxidative stress, apoptosis and promoting mTOR mediated autophagy. Life Sci. 2021 Mar;268:118992Read more>>
  • Ke Ma. et al. Articular chondrocyte-derived extracellular vesicles promote cartilage differentiation of human umbilical cord mesenchymal stem cells by activation of autophagy. J Nanobiotechnol. 2020 Dec;18(1):1-18Read more>>
  • Ling-Kun Zhang. et al. Growth factors regional patterned and photoimmobilized scaffold applied to bone tissue regeneration. J Mater Chem B. 2020 Dec;8(48):10990-11Read more>>
  • Maolin Zhang. et al. Recapitulation of cartilage/bone formation using iPSCs via biomimetic 3D rotary culture approach for developmental engineering. Biomaterials. 2020 Nov;260:120334Read more>>
  • Zubeyir Elmazoglu. et al. TLR4, RAGE, and p\JNK/JNK mediated inflammatory aggression in osteoathritic human chondrocytes are counteracted by redox\sensitive phenolic olive compounds: Comparison with ibuprofen. J Tissue Eng Regen M. 2020 Dec;14(12):1841-1857Read more>>
  • Heli Zhang. et al. FN1 promotes chondrocyte differentiation and collagen production via TGF-/PI3K/Akt pathway in mice with femoral fracture. Gene. 2021 Feb;769:145253Read more>>
  • Quan Zhang. et al. Intra-articular injection of human umbilical cord mesenchymal stem cells ameliorates monosodium iodoacetate-induced osteoarthritis in rats by inhibiting cartilage degradation and inflammation. Bone Joint Res. 2021 Mar; 10(3): 226C236Read more>>
  • Guo Zhu. et al. Exosomal MATN3 of Urine-Derived Stem Cells Ameliorates Intervertebral Disc Degeneration by Antisenescence Effects and Promotes NPC Proliferation and ECM Synthesis by Activating TGF-. Oxid Med Cell Longev. 2021;2021:5542241Read more>>
  • Alahdal M. et al. Indoleamine 2, 3 Dioxygenase 1 Impairs Chondrogenic Differentiation of Mesenchymal Stem Cells in the Joint of Osteoarthritis Mice Model.. Front Immunol. 2021 Dec;12:781185-781185Read more>>
  • Yang, Jiashu. et al. m6A-mediated upregulation of AC008 promotes osteoarthritis progression through the miR-328-3p?AQP1/ANKH axis. Exp Mol Med. 2021 Nov;:1-12Read more>>
  • Tzu-Hsiang Lin. et al. A bilineage thermosensitive hydrogel system for stimulation of mesenchymal stem cell differentiation and enhancement of osteochondral regeneration. Compos Part B-Eng. 2022 Jan;:109614Read more>>
  • Cheng, Baixiang. et al. Distinctive Roles of Wnt Signaling in Chondrogenic Differentiation of BMSCs under Coupling of Pressure and Platelet-Rich Fibrin. Tissue Engineering and Regenerative Medicine. 2022 Apr;:1-15Read more>>
  • Shengbo Sang. et al. BC enhanced photocurable hydrogel based on 3D bioprinting for nasal cartilage repair. INT J POLYM MATER. 2022 Apr 09Read more>>
  • Zhongrun Yuan. et al. Galactose Enhances Chondrogenic Differentiation of ATDC5 and Cartilage Matrix Formation by Chondrocytes. FRONT MOL BIOSCI. 2022; 9: 850778Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rabbit meniscus tissue labeled Anti-Collagen II Polyclonal Antibody, Unconjugated (bs-0709R) at 1:200, followed by conjugation to the secondary Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. and DAPI


Lane 1: Human 293T lysates; Lane 2: Human A549 lysates; Lane 3: Human MCF-7 lysates; Lane 4: Human HepG2 lysates; Lane 5: Mouse Placenta lysates; Lane 6: Human Mg63 lysates probed with Collagen II Polyclonal Antibody, Unconjugated (bs-0709R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.