DATASHEET
Host:
Rabbit
Target Protein:
NF-M
Immunogen Range:
101-200/916
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
24588
Swiss Prot:
P12839
Source:
KLH conjugated synthetic peptide derived from rat NF-M
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
PRODUCT SPECIFIC PUBLICATIONS
- Gao, Yuhua, et al. "Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells." BioMed Research International 2013 (2013).Read more>>
- Yuan, Quan, et al. "Human microvascular endothelial cell promotes the development of dorsal root ganglion neurons via BDNF pathway in a co-culture system." Bioscience, Biotechnology, and Biochemistry (2017): 1-8.Read more>>
- Wang, Xiao-feng. et al. Active constituent of Polygala tenuifolia attenuates cognitive deficits by rescuing hippocampal neurogenesis in APP/PS1 transgenic mice. Bmc Complem Altern M. 2021 Dec;21(1):1-15Read more>>
- Lei Wang. et al. Cognitive impairment is associated with BDNF-TrkB signaling mediating synaptic damage and reduction of amino acid neurotransmitters in heart failure. FASEB J. 2023 Dec;38(1):e23351Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat cochlea tissue labeled Anti-NF-M/Neurofilament M Polyclonal Antibody, Unconjugated (bs-0710R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (rat Cerebellum ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NF-M ) Polyclonal Antibody, Unconjugated (bs-0710R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.