bs-0715R [Primary Antibody]
Tubulin-Beta 3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Tubulin-Beta 3

Specificity: Based on the similarity among the proteins in this family, there is a slight chance that this antibody could recognize TBB4A and TBB6.

Immunogen Range: 31-130/444


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 10381

Swiss Prot: Q13509

Source: KLH conjugated synthetic peptide derived from human Tubulin-Beta 3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 50


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Genghua Chen. et al. Transcriptome profile analysis reveals KLHL30 as an essential regulator for myoblast differentiation. Biochem Bioph Res Co. 2021 Jun;559:84Read more>>
  • Keliang Song. et al. Knocking down Siglec-15 in osteosarcoma cells inhibits proliferation while promoting apoptosis and pyroptosis by targeting the Siglec-15/STAT3/Bcl-2 pathway. ADV MED SCI-POLAND. Adv Med Sci-Poland. 2022 Sep;67:187Read more>>
  • Yan Lin. et al. FADD phosphorylation contributes to development of renal fibrosis by accelerating epithelial-mesenchymal transition. CELL CYCLE. 2022 Oct 24Read more>>
  • Sheng Qin. et al. BmAbl1 Regulates Silk Protein Synthesis via Glutathione Metabolism in Bombyx mori. INSECTS. 2022 Nov;13(11):967Read more>>
  • Wu Yutong. et al. Osteoclast-Derived Apoptotic Bodies Inhibit Naive Cd8<sup> </sup> T Cell Activation via Siglec15 Promoting Breast Cancer Secondary Metastasis. Cell Reports Medicine. 2022 Nov 03Read more>>
  • Zhanwei Zhou. et al. Pore formingCmediated intracellular protein delivery for enhanced cancer immunotherapy. SCI ADV. 2022 NovRead more>>
  • Zhao, Binggong. et al. USP13 promotes breast cancer metastasis through FBXL14-induced Twist1 ubiquitination. CELL ONCOL. 2023 Feb;:1-17Read more>>
  • Sun Ying. et al. ETS-1-activated LINC01016 over-expression promotes tumor progression via suppression of RFFL-mediated DHX9 ubiquitination degradation in breast cancers. CELL DEATH DIS. 2023 Aug;14(8):1-12Read more>>
  • Jun Wang. et al. Network pharmacology analysis of the active ingredients of Corydalis hendersonii Hemsl. and their effects on eliminating neuroinflammation and improving motor functions in MPTP-intoxicated mice. J ETHNOPHARMACOL. 2023 Aug;:117009Read more>>
  • Genghua Chen. et al. Bulk and single-cell alternative splicing analyses reveal roles of TRA2B in myogenic differentiation. CELL PROLIFERAT. 2023 Sep;:e13545Read more>>
  • Yuxue Xu. et al. Glucagon Enhances Chemotherapy Efficacy By Inhibition of Tumor Vessels in Colorectal Cancer. ADV SCI. 2023 Dec;:2307271Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Tubulin-Beta Polyclonal Antibody, Unconjugated (bs-0715R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Rat Cerebrum lysates probed with Beta Tubulin Polyclonal Antibody, Unconjugated (bs-0715R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.