bs-0718R
[Primary Antibody]
Smad2 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Smad2
Immunogen Range: 21-120/467
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4087
Swiss Prot: Q15796
Source: KLH conjugated synthetic peptide derived from human Smad2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 52
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Goat
Others
Predicted Cross Reactive Species:
Dog
Cow
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wang, Yanqiu, et al. "Epigallocatechin-3-gallate attenuates transforming growth factor-β1 induced epithelial-mesenchymal transition via Nrf2 regulation in renal tubular epithelial cells." Biomedicine & Pharmacotherapy (2015).Read more>>
- Zhong, Di, et al. "LRG1 modulates invasion and migration of glioma cell lines through TGF-β signaling pathway." Acta Histochemica (2015).Read more>>
- Wang et al. The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-β/Smad signaling and activation of the Nrf2/HO-1 pathway. (2016) Mol.Med.Re. 14:2389-96Read more>>
- Zhou et al. Induced pluripotent stem cell-conditioned medium suppresses pulmonary fibroblast-to-myofibroblast differentiation via the inhibition of TGF-β1/Smad pathway. (2018) Int.J.Mol.Med. 41:473-484Read more>>
- Tang et al. Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways. (2016) Cell.Stress.Chaperones. 21:239-49Read more>>
- An et al. Chi-miR-4110 promotes granulosa cell apoptosis by targeting Sma- and Mad-related protein 2 (Smad2) in the caprine ovary. (2017) PLoS.One. 12:e0181162Read more>>
- Zhang et al. Adenovirus‑mediated knockdown of activin A receptor type 2A attenuates immune‑induced hepatic fibrosis in mice and inhibits interleukin‑17‑induced activation of primary hepatic stellate cells. (2018) Int.J.Mol.Med. 42:279-289Read more>>
- Chen XY et al. Pulsed Magnetic Field Stimuli Can Promote Chondrogenic Differentiation of Superparamagnetic Iron Oxide Nanoparticles-Labeled Mesenchymal Stem Cells in Rats.(2018) J Biomed Nanotechnol. 14(12):2135-2145. Read more>>
- Zhao XX et al. Inhibiting transforming growth factor-β signaling regulates in vitro maintenance and differentiation of bovine bone marrow mesenchymal stem cells.(2018)J Exp Zool B Mol Dev Evol.Nov 20.Read more>>
- Fang DP et al. Platelet‐rich plasma promotes the regeneration of cartilage engineered by mesenchymal stem cells and collagen hydrogel via the TGF‐β/SMAD signaling pathway. J Cell Physiol. 2019;1–11.Read more>>
- Ge Y et al. TCEA3 promotes differentiation of C2C12 cells via an Annexin A1‐mediated transforming growth factor‐β signaling pathway. J Cell Physiol. 2019 Jul;234(7):10554-10565.Read more>>
- Lv Y et al. Imidacloprid-induced liver fibrosis in quails via activation of the TGF-β1/Smad pathway. Sci Total Environ. 2019 Dec 6;705:135915.Read more>>
- Han B et al. Deltamethrin induces liver fibrosis in quails via activation of the TGF-β1/Smad signaling pathway. Environ Pollut. 2019 Dec 23;259:113870.Read more>>
- Yan Y et al. Inhibition of TGF-β Signaling in Gliomas by the Flavonoid Diosmetin Isolated from Dracocephalum peregrinum L. Molecules. 2020 Jan 2;25(1). pii: E192. Read more>>
- "Qi SS et al. Protective Effects of Chromium Picolinate Against Diabetic-Induced Renal Dysfunction and Renal Fibrosis in Streptozotocin-Induced Diabetic Rats. Biomolecules. 2020, 10(3), 398"Read more>>
- Zhou C et al. Remote Ischemic Preconditioning Ameliorates Renal Fibrosis After Ischemia-Reperfusion Injury via Transforming Growth Factor beta1 (TGF-β1) Signalling Pathway in Rats. Med Sci Monit. 2020 Feb 6;26:e919185. Read more>>
- Zheng HX et al. Cyanidin-3-glucoside from Black Rice Ameliorates Diabetic Nephropathy via Reducing Blood Glucose, Suppressing Oxidative Stress and Inflammation, and Regulating Transforming Growth Factor β1/Smad Expression. J Agric Food Chem. 2020 Apr 15;68(15):4399-4410. Read more>>
- Xijuan Liu et al. Chondrocyte suppression is mediated by miR\129\5p via GDF11/SMAD3 signaling in developmental dysplasia of the hip. J Orthop Res. 2020 Dec;38(12):2559-2572.Read more>>
- Huimeng Qi. et al. MicroRNA-183 exerts a protective role in lupus nephritis through blunting the activation of TGF-/Smad/TLR3 pathway via reducing Tgfbr1. Exp Cell Res. 2020 Sep;394:112138Read more>>
- Naihua Hu. et al. Forsythiae Fructuse water extract attenuates liver fibrosis via TLR4/MyD88/NF-B and TGF-/smads signaling pathways. J Ethnopharmacol. 2020 Nov;262:113275Read more>>
- Huajun Wang. et al. LncRNA NEAT1 promotes proliferation, migration, invasion and epithelial-mesenchymal transition process in TGF-2-stimulated lens epithelial cells through regulating the miR-486-5p/SMAD4 axis. Cancer Cell Int. 2020 Dec;20(1):1-12Read more>>
- Fei Yin. et al. Effect of Human Umbilical Cord Mesenchymal Stem Cells Transfected with HGF on TGF-1/Smad Signaling Pathway in Carbon Tetrachloride-Induced Liver Fibrosis Rats. Stem Cells Dev. 2020 Oct;29(21):1395-1406Read more>>
- Zheng Wu. et al. FOXD3 suppresses epithelialCmesenchymal transition through direct transcriptional promotion of SMAD7 in esophageal squamous cell carcinoma. 2021 Sep 22Read more>>
- Feng Wang. et al. Metformin reduces myogenic contracture and myofibrosis induced by rat knee joint immobilization via AMPK-mediated inhibition of TGF-_1/Smad signaling pathway. CONNECT TISSUE RES. 2022 Jun 2Read more>>
- Zhou, Jing. et al. Role of magnesium-doped calcium sulfate and _-tricalcium phosphate composite ceramics in macrophage polarization and osteo-induction. ODONTOLOGY. 2022 Jun;:1-12Read more>>
- Sheng-Zhong Han. et al. miR-455-3p Is Negatively Regulated by Myostatin in Skeletal Muscle and Promotes Myoblast Differentiation. J AGR FOOD CHEM. 2022;70(33):1012110133Read more>>
- Zhao, Xin. et al. NFK prevent postoperative abdominal adhesion through downregulating the TGF-1 signaling pathway. MOL BIOL REP. 2022 Nov;:1-1Read more>>
- Li Zhang. et al. Erxian herbal pair enhances bone formation in infected bone nonunion models and attenuates lipopolysaccharide-induced osteoblastinhibition by regulating miRNA-34a-5p. BIOENGINEERED. 2022;13(6):14339-14356Read more>>
- Xiaokereti, Jiasuoer. et al. Renal denervation alleviates chronic obstructive sleep apnea-induced atrial fibrillation via inhibition of atrial fibrosis and sympathetic hyperactivity. SLEEP BREATH. 2023 Feb;:1-14Read more>>
- Niu YangYang. et al. The significance of spread through air spaces in the prognostic assessment model of stage I lung adenocarcinoma and the exploration of its invasion mechanism. J CANCER RES CLIN. 2023 Mar;:1-14Read more>>
- Xiaoliang Zhou. et al. Ursolic acid inhibits human dermal fibroblasts hyperproliferation, migration, and collagen deposition induced by TGF- via regulating the Smad2/3 pathway. GENE. 2023 May;867:147367Read more>>
- Huang Shu. et al. Targeting nano-regulator based on metalCorganic frameworks for enhanced immunotherapy of bone metastatic prostate cancer. CANCER NANOTECHNOL. 2023 Dec;14(1):1-15Read more>>
- Leihao Hu. et al. Identification of the Active Compounds in the Yi-Fei-San-Jie Formula Using A Comprehensive Strategy Based on Cell Extraction/UPLC-MS/MS, Network Pharmacology, and Molecular Biology Techniques. PHYTOMEDICINE. 2023 Apr;:154843Read more>>
- Genghua Chen. et al. Bulk and single-cell alternative splicing analyses reveal roles of TRA2B in myogenic differentiation. CELL PROLIFERAT. 2023 Sep;:e13545Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse kidney lysates probed with Anti-Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse placenta lysates probed with Anti-Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Heart lysates; Lane 3: Mouse Testis lysates; Lane 4: Mouse Kidney lysates; Lane 5: Mouse Placenta lysates; Lane 6: Rat Cerebrum lysates; Lane 7: Rat Heart lysates; Lane 8: Rat Testis lysates; Lane 9: Rat Kidney lysates; Lane 10: Human Hela cell lysates; Lane 11: Human HT1080 cell lysates; Lane 12: Human Jurkat cell lysates; Lane 13: Mouse RAW264.7 cell lysates; Lane 14: Human HL-60 cell lysates probed with Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.