bs-0723R [Primary Antibody]
p63 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: p63

Immunogen Range: 370-420/680

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8626

Swiss Prot: Q9H3D4

Source: KLH conjugated synthetic peptide derived from human p63 protein

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months.

Background: Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yang, Rong-Hua, et al. "An improved method for the isolation and culture of rat epidermal stem cells." International Journal of Clinical and?Experimental Pathology6.11 (2013): 2529-2534.Read more>>
  • Quantius J, Schmoldt C, Vazquez-Armendariz AI, Becker C, El Agha E, Wilhelm J, et al. (2016) Influenza Virus Infects Epithelial Stem/Progenitor Cells of the Distal Lung: Impact on Fgfr2b-Driven Epithelial Repair. PLoS Pathogen 12(6): e1005544.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat heart labeled with Rabbit Anti P63 protein/P51A Polyclonal Antibody, Unconjugated (bs-0723R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: A431 Cell lysates; Probed with P63 Polyclonal Antibody, unconjugated (bs-0723R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.