bs-0725R [Primary Antibody]
Estrogen Receptor alpha Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Estrogen Receptor alpha

Immunogen Range: 501-600/600


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24890

Swiss Prot: P06211

Source: KLH conjugated synthetic peptide derived from rat ER-alpha

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 67


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ye, Zonghuang, et al. "Electrochemical biosensor for the nuclear factor kappa B using a gold nanoparticle-assisted dual signal amplification method." Microchimica Acta (2013): 1-7.Read more>>
  • Zuo, Zhenghong, et al. "Chronic Exposure to Tributyltin Chloride Induces Pancreatic Islet cell Apoptosis and Disrupts Glucose Homeostasis in male mice." Environmental Science & Technology (2014).Read more>>
  • Wu et al. Downregulation of G?protein?coupled receptor 30 in the hippocampus attenuates the neuroprotection of estrogen in the critical period hypothesis. (2018) Mol.Med.Rep. 17:5716-5725Read more>>
  • Liu R et al. ERβ modulates genistein's cisplatin-enhancing activities in breast cancer MDA-MB-231 cells via P53-independent pathway. Mol Cell Biochem. 2019 Feb 8.Read more>>
  • Liu R et al. ERβ modulates genistein's cisplatin-enhancing activities in breast cancer MDA-MB-231 cells via P53-independent pathway. Mol Cell Biochem. 2019 Feb 8.Read more>>
  • Jia-Hua Zhenget al. Quantification of the CM-Dil-labeled Human umbilical cord mesenchymal stem cells migrated to the dual injured uterus in SD rat. Stem Cell Res Ther. 2020 Jul 13;11(1):280.Read more>>
  • Keiichi Hiramoto. et al. Role of Momordica charantia in preventing the natural aging process of skin and sexual organs in mice. Dermatol Ther. 2020 Nov;33(6):e14243Read more>>
  • Yuan, Jie. et al. Comparison of the efficacy of gossypol acetate enantiomers in rats with uterine leiomyoma. J NAT MED-TOKYO. 2022 Aug;:1-12Read more>>
  • Yudong Yin. et al. (4-Picolylamino)-17-Estradiol derivative and analogues induce apoptosis with death receptor trail R2/DR5 in MCF-7. CHEM-BIOL INTERACT. 2022 Nov;:110286Read more>>
  • Theresa K?nig. et al. Multi-Parameter Analysis of Disseminated Tumor Cells (DTCs) in Early Breast Cancer Patients with Hormone-Receptor-Positive Tumors. CANCERS. 2023 Jan;15(3):568Read more>>
VALIDATION IMAGES

Rat brain lysates probed with Anti ERα/ESR1 Polyclonal Antibody, Unconjugated (bs-0725R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 67kD. Observed band size:67kD.


MCF-7 cells probed with Estrogen Receptor alpha Antibody, unconjugated (bs-0725R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)


Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Estrogen Receptor alpha Polyclonal Antibody, Unconjugated (bs-0725R) at 1:200 overnight at 4°C, DAB staining.


Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Estrogen Receptor alpha) polyclonal Antibody, Unconjugated (bs-0725R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.