bs-0741R
[Primary Antibody]
CDKN1A Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CDKN1A
Immunogen Range: 101-164/164
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 12575
Swiss Prot: P38936
Source: KLH conjugated synthetic peptide derived from human CDKN1A
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex (By similarity).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 18
Cross Reactive Species:
Human
Mouse
Rat
Sheep
Predicted Cross Reactive Species:
Dog
Cow
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Chen, Jie, et al. "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." BMC cancer 12.1 (2012): 464.Read more>>
- Li, Lv, et al. "Lycorine induces cell-cycle arrest in the G0/G1 phase in K562 cells via HDAC inhibition." Cancer cell international 12.1 (2012): 1-6.Read more>>
- Jindou Jiang, Yong Miao, et al. "DAPT in the control of human hair follicle stem cell proliferation and differentiation." Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii 31.4 (2014): 201.Read more>>
- Yang et al. RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells. (2011) J.Biol.Che. 286:11865-74Read more>>
- Yang et al. RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells. (2011) J.Biol.Che. 286:11865-74Read more>>
- Shen S et al. Transcription factor early growth response-1 plays an oncogenic role in salivary gland pleomorphic adenoma. Biotechnol Lett. 2019 Nov 30.Read more>>
- Niu K et al. Pepsin promotes laryngopharyngeal neoplasia by modulating signaling pathways to induce cell proliferation. PLoS One. 2020 Jan 15;15(1):e0227408.Read more>>
- Shi L et al. Effects of selenium on the proliferation and apoptosis of sheep spermatogonial stem cells in vitro. Animal Reproduction Science, 2020, 106330. Read more>>
- Gong Y et al. Smad3 C-terminal phosphorylation site mutation attenuates the hepatoprotective effect of salvianolic acid B against hepatocarcinogenesisFood Chem Toxicol.2021 Jan;147:111912.Read more>>
- Yunping Lu. et al. Peroxiredoxin1 Knockdown Inhibits Oral Carcinogenesis via Inducing Cell Senescence Dependent on Mitophagy. Oncotargets Ther. 2021; 14: 239C251Read more>>
- Feng Cui. et al. ML216 Prevents DNA Damage-Induced Senescence by Modulating DBC1–BLM Interaction. CELLS-BASEL. 2023 Jan;12(1):145Read more>>
- Yue Zhang. et al. Dietary selenium excess affected spermatogenesis via DNA damage and telomere-related cell senescence and apoptosis in mice. FOOD CHEM TOXICOL. 2023 Jan;171:113556Read more>>
- Keiichi Hiramoto. et al. Bacillus coagulans (species of lactic acid-forming Bacillus bacteria) ameliorates azoxymethane and dextran sodium sulfate-induced colon cancer in mice. J FUNCT FOODS. 2023 Jan;100:105406Read more>>
- Fang Wu. et al. Ribosomal protein L31 (RPL31) inhibits the proliferation and migration of gastric cancer cells. HELIYON. 2023 Jan;:e13076Read more>>
- Jinxin Ma. et al. Bioinformatics and Integrative Experimental Method to Identifying and Validating Co-Expressed Ferroptosis-Related Genes in OA Articular Cartilage and Synovium. J INFLAMM RES. 2024 Feb 11Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human laryngeal squamous cell carcinoma labeled Anti-P21/CDKN1A Polyclonal Antibody, Unconjugated (bs-0741R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CDKN1A Polyclonal Antibody, Unconjugated (bs-0741R) at 1:200 overnight at 4°C, DAB staining.
Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CDKN1A/p21) Polyclonal Antibody, Unconjugated (bs-0741R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CDKN1A Polyclonal Antibody, Unconjugated (bs-0741R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CDKN1A Polyclonal Antibody, Unconjugated (bs-0741R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CDKN1A Polyclonal Antibody, Unconjugated (bs-0741R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CDKN1A Polyclonal Antibody, Unconjugated (bs-0741R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CDKN1A Polyclonal Antibody, Unconjugated (bs-0741R) at 1:200 overnight at 4°C, DAB staining.