Host: Rabbit
Target Protein: CDKN1B
Immunogen Range: 101-198/198
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1027
Swiss Prot: P46527
Source: KLH conjugated synthetic peptide derived from human CDKN1B
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 22
Cross Reactive Species:
Human
Mouse
Rat
Chicken
Others
Predicted Cross Reactive Species:
Dog
Sheep
Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Sun, Yan, et al. "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." Theriogenology (2014).Read more>>
- Gao, LiLi, et al. "Protein-Binding Function of RNA-Dependent Protein Kinase Promotes Proliferation through TRAF2/RIP1/NF-κB/c-Myc Pathway in Pancreatic β cells." Molecular Medicine 21.1 (2015): 154.Read more>>
- Akizuki, Risa, et al. "Claudin-5,− 7, and− 18 suppress proliferation mediated by inhibition of phosphorylation of Akt in human lung squamous cell carcinoma." Biochimica et Biophysica Acta (BBA)-Molecular Cell Research (2016).Read more>>
- Guo, Fangzi, et al. "Endosulfan induces apoptosis by activating the negative regulation pathway of cell cycle and death receptor pathway in spermatogenic cells." Toxicology Research (2017).Read more>>
- Abdalla, BA., et al. “A Novel Dnmt3a1 Transcript Inhibits Adipogenesis.” Frontiers in Physiology (2018); 9.Read more>>
- Abdalla et al. A Novel Dnmt3a1 Transcript Inhibits Adipogenesis. (2018) Front.Physiol. 9:1270Read more>>
- Yang K et al. MicroRNA‐222‐3p participates in the development of oral squamous cell carcinoma by targeting CDKN1B. J Oral Pathol Med. 2019 Dec 16.Read more>>
- Liu A et al. Down-regulation of NTSR3 inhibits growth, metastasis, and PI3K/AKT and MAPK signaling pathways in colorectal cancer cells. Biochem Cell Biol. 2020 Mar 3.Read more>>
- Ali B et al. Control of preadipocyte proliferation, apoptosis and early adipogenesis by the forkhead transcription factor FoxO6Life Sci.2021 Jan 15;265:118858.Read more>>
- Cuifang Chang. et al. The orphan GPR50 receptor interacting with T_RI induces G1/S-phase cell cycle arrest via Smad3-p27/p21 in BRL-3A cells. BIOCHEM PHARMACOL. 2022 Aug;202:115117Read more>>
- Wang Haining. et al. Integrative analysis identifies oxidative stress biomarkers in non-alcoholic fatty liver disease via machine learning and weighted gene co-expression network analysis. FRONT IMMUNOL. 2024 Feb;15Read more>>
Formalin-fixed and paraffin embedded human ovary carcinoma labeled Anti-CDKN1B/P27kip1 Polyclonal Antibody, Unconjugated (bs-0742R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
MCF-7 cells were stained with CDKN1B Polyclonal Antibody, Unconjugated(bs-0742R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
RSC96 probed with CDKN1B Polyclonal Antibody, Unconjugated (bs-0742R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Mouse lung lysates probed with CDKN1B Polyclonal Antibody, unconjugated (bs-0742R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Lane 1: Hela cell lysates; Lane 2: Hela KO CDKN1B lysates probed with CDKN1B Polyclonal Antibody, Unconjugated (bs-0742R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: U2os cell lysates probed with CDKN1B Polyclonal Antibody, Unconjugated (bs-0742R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.