bs-0747R-A350 [Conjugated Primary Antibody]
RANKL Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: RANKL

Immunogen Range: 210-317/317


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8600

Swiss Prot: O14788

Source: KLH conjugated synthetic peptide derived from human RANKL

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Conjugation: ALEXA FLUOR® 350

Excitation/ Emission: 346nm/442nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 35


Cross Reactive Species: Human
Mouse
Rat
Rabbit

Predicted Cross Reactive Species: Dog

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li, Ping. "Efficacy and Safety of Echinacoside in a Rat Osteopenia Model." Evidence-Based Complementary and Alternative Medicine 2013 (2013).Read more>>
  • Sun, Jiabing, et al. "A Crucial Role of IL-17 in Bone Resorption During Rejection of Fresh Bone Xenotransplantation in Rats." Cell Biochemistry and Biophysics: 1-7.Read more>>
  • Yu, Xijiao, et al. "Expression of neuropeptides and bone remodeling-related factors during periodontal tissue regeneration in denervated rats." Journal of Molecular Histology: 1-9.Read more>>
  • Zhu, Wenjing, et al. "Effect of PI3K/Akt Signaling Pathway on the Process of Prostate Cancer Metastasis to Bone." Cell Biochemistry and Biophysics (2015): 1-7.Read more>>
  • Ota, Takehiro, et al. "Expression of colony-stimulating factor 1 is associated with occurrence of osteochondral change in pigmented villonodular synovitis."Tumor Biology (2015): 1-7.Read more>>
  • Li, Xianxian, et al. "Oral administration of 5-Hydroxytryptophan aggravated periodontitis-induced alveolar bone loss in rats." Archives of Oral Biology(2015).Read more>>
  • Zhang, Yuanyu, et al. "Mycobacterium tuberculosis 10-kDa co-chaperonin regulates the expression levels of receptor activator of nuclear factor-κB ligand and osteoprotegerin in human osteoblasts." Experimental and Therapeutic Medicine.Read more>>
  • Chen, Zhiguang, et al. "Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway."International Journal of Molecular Medicine.Read more>>
  • Xiao, Wanan, et al. "Bone fracture healing is delayed in splenectomic rats." Life Sciences (2016).Read more>>
  • Zhang, Xiaonan, et al. "Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway." Chemico-Biological Interactions 256 (2016): 188-197.Read more>>
  • Dolci, Gabriel Schmidt, et al. "Atorvastatin-induced osteoclast inhibition reduces orthodontic relapse." American Journal of Orthodontics and Dentofacial Orthopedics 151.3 (2017): 528-538.Read more>>
  • He, Ming, et al. "Effect of glucocorticoids on osteoclast function in a mouse model of bone necrosis." Molecular medicine reports 14.2 (2016): 1054-1060.Read more>>
  • Yu, X., et al. "Denervation effectively aggravates rat experimental periodontitis." Journal of Periodontal Research (2017).Read more>>
  • Zhan, Fu‐Liang, Xin‐Yang Liu, and Xing‐Bo Wang. "The Role of MicroRNA‐143‐5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway." Journal of Cellular Biochemistry (2017).Read more>>
  • Chen, Helin, et al. "Intermittent administration of parathyroid hormone ameliorated alveolar bone loss in experimental periodontitis in streptozotocin-induced diabetic rats." Archives of Oral Biology (2017).Read more>>
  • Zhan et al. The Role of MicroRNA-143-5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway. (2018) J.Cell.Biochem. 119:536-546Read more>>
  • Peng W et al.Expression of osteoprotegerin and receptor activator for the nuclear factor-κB ligand in XACB/LV-bFGF/MSCs transplantation for repair of rabbit femoral head defect necrosis.(2018) J. Cell. Biochem. Oct 18Read more>>
  • Chen Y et al. Moxibustion of Zusanli (ST36) and Shenshu (BL23) Alleviates Cartilage Degradation through RANKL/OPG Signaling in a Rabbit Model of Rheumatoid Arthritis. Evid Based Complement Alternat Med. 2019 Jan 3;2019:6436420.Read more>>
  • Yu X et al. Substance P aggravates periodontitis by upregulating HIF-1α and RANKL/OPG ratio. BMC Oral Health. 2019.Read more>>
  • Yan KX et al. Substance P participates in periodontitis by upregulating HIF-1α and RANKL/OPG ratio. BMC Oral Health. 2019 Dec.Read more>>
  • Zhang C et al. FOXO1 Mediates Advanced Glycation End Products Induced Mouse Osteocyte-Like MLO-Y4 Cell Apoptosis and Dysfunctions. Journal of Diabetes Research.2019, Article ID 6757428.Read more>>
  • Kang J et al. Matrine protects retinal ganglion cells from apoptosis in experimental optic neuritis. 29 August 2019, PREPRINT (Version 1) available at Research Square.Read more>>
  • Zou MX et al. Clinical Impact of the Immune Microenvironment in Spinal Chordoma: Immunoscore as an Independent Favorable Prognostic Factor.Neurosurgery. 2018 Jul 19. Read more>>
  • Yang Y et al. Ganoderma lucidum Immune Modulator Protein rLZ-8 Could Prevent and Reverse Bone Loss in Glucocorticoids-Induced Osteoporosis Rat Model. Front Pharmacol. 2020 May 19;11:731.Read more>>
  • Teng X et al. Effects of Low Dietary Phosphorus on Tibia Quality and Metabolism in Caged Laying Hens. Prev Vet Med. 2020 Aug;181:105049.Read more>>
  • Daisuke Nishida. et al. RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp. Sci Rep-Uk. 2021 Feb;11(1):1-12Read more>>
  • Hongyi Qu. et al. The effects of vasoactive intestinal peptide on RANKL-induced osteoclast formation. Ann Transl Med. 2021 Jan; 9(2): 127Read more>>
  • Yongkui Wang. et al. Daphnetin ameliorates glucocorticoid-induced osteoporosis via activation of Wnt/GSK-3/-catenin signaling. Toxicol Appl Pharm. 2020 Dec;409:115333Read more>>
  • Yumi Sakamoto. et al. High-mobility group box 1 induces bone destruction associated with advanced oral squamous cancer via RAGE and TLR4. Biochem Bioph Res Co. 2020 Oct;531:422Read more>>
  • Wang Tong. et al. Acupotomy Contributes to Suppressing Subchondral Bone Resorption in KOA Rabbits by Regulating the OPG/RANKL Signaling Pathway. Evid-Based Compl Alt. 2021;2021:8168657Read more>>
  • Yutao Cui. et al. Functionalized anti-osteoporosis drug delivery system enhances osseointegration of an inorganicCorganic bioactive interface in osteoporotic microenvironment. Mater Design. 2021 Aug;206:109753Read more>>
  • aixiang Wang. et al. Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro. J Zhejiang Univ-Sc B. 2021 May;22(5):410-42Read more>>
  • Shunichiro Matsuoka. et al. Undifferentiated Pleomorphic Sarcoma of Soft Tissue with Multinucleated Giant Cells with Osteogenic Phenotypes: A Mimicker of Malignant Giant Cell Tumor of Soft Tissue. 2021 Jul 21Read more>>
  • Xinkun Shen. et al. Improvement of aqueous stability and anti-osteoporosis properties of Zn-MOF coatings on titanium implants by hydrophobic raloxifene. Chem Eng J. 2021 Oct;:133094Read more>>
  • Hiroki Ehara. et al. Role of tissue factor in delayed bone repair induced by diabetic state in mice. Plos One. 2021 Dec;16(12):e0260754Read more>>
  • Qiusheng Shan. et al. Significance of cancer stroma for bone destruction in oral squamous cell carcinoma using different cancer stroma subtypes. Oncol Rep. 2022 Apr;47(4):1-13Read more>>
  • Lingli Ding. et al. Ginsenoside Compound K Enhances Fracture Healing <i>via</i> Promoting Osteogenesis and Angiogenesis.. FRONT PHARMACOL. 2022 Apr;13:855393-855393Read more>>
  • Huijuan Shen. et al. microRNA-146a mediates distraction osteogenesis via bone mesenchymal stem cell inflammatory response. ACTA HISTOCHEM. 2022 Aug;124:151913Read more>>
  • Qiusheng Shan. et al. Investigation of bone invasion and underlying mechanisms of oral cancer using a cell line?derived xenograft model. ONCOL LETT. 2022 Nov;24(5):1-12Read more>>
  • Albert chun-shuo Huang. et al. NF-&kappa;B Decoy ODN-Loaded Poly(Lactic-co-glycolic Acid) Nanospheres Inhibit Alveolar Ridge Resorption. INT J MOL SCI. 2023 Jan;24(4):3699Read more>>
  • Yang Liu. et al. Isochlorogenic Acid C Restrains Erk/JNK/NF-B Signaling to Alleviate Inflammatory Response and Promote Cell Apoptosis. J FOOD BIOCHEM. 2023;2023:5547108Read more>>
  • Xue Jiang. et al. Regulation of Wnt Signaling Pathway by Costic Acid Derivative, An Efficient Strategy for Treatment of Glucocorticoid-Induced Osteoporosis in Rat Model. CHEMISTRYSELECT. 2023 Apr;8(13):e202204912Read more>>
  • Cheng Lanlan. et al. Liraglutide attenuates palmitate-induced apoptosis via PKA/-catenin/Bcl-2/Bax pathway in MC3T3-E1 cells. N-S ARCH PHARMACOL. 2023 Jul;:1-13Read more>>
  • Hongxing Zheng. et al. Chromium Picolinate Regulates Bone Metabolism and Prevents Bone Loss in Diabetic Rats. MOLECULES. 2024 Jan;29(5):924Read more>>
  • Rintanalert Duangtawan. et al. SDF-1 involvement in orthodontic tooth movement after tooth extraction. SCI REP-UK. 2024 Feb;14(1):1-15Read more>>
VALIDATION IMAGES