bs-0756R-A647 [Conjugated Primary Antibody]
Vimentin Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Vimentin

Specificity: KO-Validated

Immunogen Range: 371-466/466


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7431

Swiss Prot: P08670

Source: KLH conjugated synthetic peptide derived from human Vimentin

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Conjugation: ALEXA FLUOR® 647

Excitation/ Emission: 650nm/665nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 53


Cross Reactive Species: Human
Mouse
Rat
Bovine
Pig
Others
Monkey

Predicted Cross Reactive Species: Cow
Chicken
Goat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Yueqin, et al. "Hypoxia-Inducible Factor-1alpha and MAPK Co-Regulate Activation of Hepatic Stellate Cells upon Hypoxia Stimulation." PLoS ONE 8.9 (2013).Read more>>
  • Hu, Bin, et al. "IFN‐γ Inhibits Osteopontin Expression in Human Decidual Stromal Cells and can be Attenuated by 1α, 25‐Dihydroxyvitamin D3." American Journal of Reproductive Immunology 68.4 (2012): 353-361.Read more>>
  • Liu, Yang, et al. "Down-regulation of< i> Wnt10a</i> affects odontogenesis and proliferation in mesenchymal cells." Biochemical and Biophysical Research Communications (2013).Read more>>
  • Fu, Min, et al. "Effects of Ureaplasma urealyticum lipid-associated membrane proteins on rheumatoid arthritis synovial fibroblasts." Journal of International Medical Research 41.5 (2013): 1655-1670.Read more>>
  • Li, Yingli, et al. "Differences between Niche Cells and Limbal Stromal Cells in Maintenance of Corneal Limbal Stem Cells." Investigative Ophthalmology & Visual Science (2014): IOVS-13Read more>>
  • Sottnik, Joseph L., et al. "Osteocytes Serve as a Progenitor Cell of Osteosarcoma." Journal of Cellular Biochemistry (2014).Read more>>
  • Wang, Jing, et al. "Immortalized porcine intestinal epithelial cell cultures susceptible to porcine rotavirus infection." Journal of Virological Methods (2014).Read more>>
  • Pan, Yan, et al. "Enoxaparin Sensitizes Human Non-small-cell Lung Carcinomas to Gefitinib by Inhibiting DOCK1 Expression, Vimentin Phosphorylation and Akt Activation." Molecular pharmacology (2014): mol-114.Read more>>
  • Tong, Weihua, et al. "Sorcin Enhances Metastasis and Promotes Epithelial-to-Mesenchymal Transition of Colorectal Cancer." Cell Biochemistry and Biophysics (2015): 1-7.Read more>>
  • Cheng, Gangwei, et al. "Direct Effects of Bevacizumab on Rat Conjunctival Fibroblast." Cell Biochemistry and Biophysics: 1-6.Read more>>
  • Deng, Boya, et al. "MicroRNA-142-3p inhibits cell proliferation and invasion of cervical cancer cells by targeting FZD7." Tumor Biology (2015): 1-9.Read more>>
  • Fernández-Ferreiro, Anxo, et al. "In vitro and in vivo ocular safety and eye surface permanence determination by direct and Magnetic Resonance Imaging of ion-sensitive hydrogels based on Gellan gum and kappa-carrageenan."European Journal of Pharmaceutics and Biopharmaceutics (2015).Read more>>
  • Karim, A. S., et al. "Nox2 Is a Mediator of Ischemia Reperfusion Injury."American Journal of Transplantation (2015).Read more>>
  • Wang, Li-Ping, et al. "Angiotensin II upregulates K Ca 3.1 channels and stimulates cell proliferation in rat cardiac fibroblasts." Biochemical pharmacology 85.10 (2013): 1486-1494.Read more>>
  • Su, Jing, and Hong Li. "RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells." International Journal of Clinical and Experimental Pathology 8.9 (2015): 10760-10767.Read more>>
  • Huang, Chao, and Bin Wen. "Phenotype transformation of immortalized NCM460 colon epithelial cell line by TGF-β1 is associated with chromosome instability." Molecular Biology Reports: 1-10.Read more>>
  • Sucre, Jennifer MS, et al. "A three-dimensional human model of the fibroblast activation that accompanies bronchopulmonary dysplasia identifies Notch-mediated pathophysiology." American Journal of Physiology-Lung Cellular and Molecular Physiology 310.10 (2016): L889-L898.Read more>>
  • Chen, Jia, et al. "Biological characterization of metanephric mesenchymal stem cells from the Beijing duck." Experimental and Therapeutic Medicine 11.2 (2016): 439-447.Read more>>
  • Wilkinson, Dan C., et al. "Development of a Three-Dimensional Bioengineering Technology to Generate Lung Tissue for Personalized Disease Modeling." Stem Cells Translational Medicine (2016): sctm-2016.Read more>>
  • Yap, Chan Choo, et al. "Different doublecortin (DCX) patient alleles show distinct phenotypes in cultured neurons: evidence for divergent loss-of-function and off-pathway cellular mechanisms." Journal of Biological Chemistry (2016): jbc-M116.Read more>>
  • Li, Junqin, et al. "Fatty acid synthase mediates the epithelial-mesenchymal transition of breast cancer cells." International Journal of Biological Sciences10.2 (2014): 171.Read more>>
  • Pagani, Isabel, et al. "Human Endometrial Stromal Cells Are Highly Permissive To Productive Infection by Zika Virus." bioRxiv (2016): 077305.Read more>>
  • Xiao, Jiajia, et al. "Isolation of Bovine Skin-Derived Precursor Cells and Their Developmental Potential After Nuclear Transfer." Cellular Reprogramming (Formerly" Cloning and Stem Cells") 18.6 (2016): 411-418.Read more>>
  • Daverey, Amita, and Sandeep K. Agrawal. "Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes." Neuroscience 333 (2016): 92-103.Read more>>
  • Smith, Elizabeth E., et al. "Developing a biomimetic tooth bud model." Journal of Tissue Engineering and Regenerative Medicine (2017).Read more>>
  • Khuder, Saja S. Regulation of Expression of CEACAM1 and Functional Correlation in Metabolic Diseases. Diss. University of Toledo, 2016.Read more>>
  • Huang, Chao, and Bin Wen. "Phenotype transformation of immortalized NCM460 colon epithelial cell line by TGF-β1 is associated with chromosome instability." Molecular Biology Reports 43.10 (2016): 1069-1078.Read more>>
  • Wang, Jing, et al. "Phosphorylation-dependent regulation of ALDH1A1 by Aurora kinase A: insights on their synergistic relationship in pancreatic cancer." BMC biology 15.1 (2017): 10.Read more>>
  • Tanos, T., et al. "Isolation of putative stem cells present in human adult olfactory mucosa." PloS one 12.7 (2017): e0181151.Read more>>
  • Park, Song, et al. "Critical roles of hMAGEA2 in induced pluripotent stem cell pluripotency, proliferation, and differentiation." Cell Biochemistry and Function (2017).Read more>>
  • Pan et al. Roles of vimentin and 14-3-3 zeta/delta in the inhibitory effects of heparin on PC-3M cell proliferation and B16-F10-luc-G5 cells metastasis. (2012) Acta.Pharmacol.Si. 33:798-808Read more>>
  • Gao et al. Hes1 promotes cell proliferation and migration by activating Bmi-1 and PTEN/Akt/GSK3β pathway in human colon cancer. (2015) Oncotarge. 6:38667-80Read more>>
  • Wang et al. 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts. (2017) Med.Sci.Moni. 23:46-56Read more>>
  • Chan et al. Investigating the Radioresistant Properties of Lung Cancer Stem Cells in the Context of the Tumor Microenvironment. (2016) Radiat.Res. 185:169-81Read more>>
  • Djamali et al. Nox2 and Cyclosporine-Induced Renal Hypoxia. (2016) Transplantation. 100:1198-210Read more>>
  • Fagundes et al. Binge Drinking of Ethanol during Adolescence Induces Oxidative Damage and Morphological Changes in Salivary Glands of Female Rats. (2016) Oxid.Med.Cell.Longe. 2016:7323627Read more>>
  • Yap et al. Different Doublecortin (DCX) Patient Alleles Show Distinct Phenotypes in Cultured Neurons: EVIDENCE FOR DIVERGENT LOSS-OF-FUNCTION AND "OFF-PATHWAY" CELLULAR MECHANISMS. (2016) J.Biol.Chem. 291:26613-26626Read more>>
  • Khayat et al. GelMA-Encapsulated hDPSCs and HUVECs for Dental Pulp Regeneration. (2017) J.Dent.Res. 96:192-199Read more>>
  • Wang et al. The Aurora-A-Twist1 axis promotes highly aggressive phenotypes in pancreatic carcinoma. (2017) J.Cell.Sci. 130:1078-1093Read more>>
  • Wang et al. Anti-proliferative activities of finasteride in benign prostate epithelial cells require stromal fibroblasts and c-Jun gene. (2017) PLoS.One. 12:e0172233Read more>>
  • Pagani et al. Human Endometrial Stromal Cells Are Highly Permissive To Productive Infection by Zika Virus. (2017) Sci.Rep. 7:44286Read more>>
  • Zhou et al. GDC-0449 improves the antitumor activity of nano-doxorubicin in pancreatic cancer in a fibroblast-enriched microenvironment. (2017) Sci.Rep. 7:13379Read more>>
  • Tang et al. Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways. (2016) Cell.Stress.Chaperones. 21:239-49Read more>>
  • Zhou et al. Induced Pluripotent Stem Cells Inhibit Bleomycin-Induced Pulmonary Fibrosis in Mice through Suppressing TGF-β1/Smad-Mediated Epithelial to Mesenchymal Transition. (2016) Front.Pharmacol. 7:430Read more>>
  • Shan et al. Wnt/β-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells. (2016) Int.J.Clin.Exp.Pathol. 8:12357-67Read more>>
  • Fei et al. The number of polyploid giant cancer cells and epithelial-mesenchymal transition-related proteins are associated with invasion and metastasis in human breast cancer. (2015) J.Exp.Clin.Cancer.Res. 34:158Read more>>
  • Wang et al. Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. (2016) Sci.Rep. 6:36543Read more>>
  • Monteiro et al. Dental cell sheet biomimetic tooth bud model. (2016) Biomaterials. 106:167-79Read more>>
  • Wang et al. Expression and clinical significance of autophagic protein LC3B and EMT markers in gastric cancer. (2018) Cancer.Manag.Res. 10:1479-1486Read more>>
  • Tarique I et al. LIPOPHAGY: a novel form of steroidogenic activity within the LEYDIG cell during the reproductive cycle of turtle. Reprod Biol Endocrinol. 2019 Feb 9;17(1):19. Read more>>
VALIDATION IMAGES