bs-0780R
[Primary Antibody]
IL-8 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: IL-8
Immunogen Range: 31-99/99
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3576
Swiss Prot: P10145
Source: KLH conjugated synthetic peptide derived from human IL-8
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-1-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 8-Nov
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Predicted Cross Reactive Species:
Cow
Sheep
Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Ma, Yanqin, et al. "Inflammatory responses induced by fluoride and arsenic at toxic concentration in rabbit aorta." Archives of toxicology 86.6 (2012): 849-856.Read more>>
- Lu, Yongping, et al. "Assessment of atherosclerotic plaques in the rabbit abdominal aorta with interleukin-8 monoclonal antibody-targeted ultrasound microbubbles." Molecular biology reports (2013): 1-10.Read more>>
- Zhang, Tao, et al. "CXCL1 mediates obesity-associated adipose stromal cell trafficking and function in the tumour microenvironment." Nature Communications 7 (2016).Read more>>
- Huang, Qi. "IL-17 Promotes Angiogenic Factors IL-6, IL-8, and Vegf Production via Stat1 in Lung Adenocarcinoma." Scientific Reports 6 (2016).Read more>>
- Xu et al. Extracts of Bauhinia championii (Benth.) Benth. attenuate the inflammatory response in a rat model of collagen-induced arthritis. (2016) Mol.Med.Re. 13:4167-74Read more>>
- Ding S et al. Inflammatory cytokines tumor necrosis factor-α and interleukin-8 enhance airway smooth muscle contraction by increasing L-type Ca channel expression.(2018) Clin. Exp. Pharmacol. Physiol.Sep 10Read more>>
- Verma S et al. Non-invasive oral cancer detection from saliva using zinc oxide–reduced graphene oxide nanocomposite based bioelectrode. MRS Communications,2019, 1–8. Read more>>
- Yang W et al. The Effect of Low and High Dose Deoxynivalenol on Intestinal Morphology, Distribution, and Expression of Inflammatory Cytokines of Weaning Rabbits. Toxins (Basel). 2019 Aug 13;11(8). pii: E473.Read more>>
- Xin Fan. et al. Anti-Colon Cancer Activity of Novel Peptides Isolated from In Vitro Digestion of Quinoa Protein in Caco-2 Cells. Foods. 2022 Jan;11(2):194Read more>>
- Mingsheng Lyu. et al. Molecular Mechanism Underlying Effects of Wumeiwan on Steroid-Dependent Asthma: A Network Pharmacology, Molecular Docking, and Experimental Verification Study. Drug Des Dev Ther. 2022; 16: 909929Read more>>
- Junfeng Qiu. et al. FOXS1 Promotes Tumor Progression by Upregulating CXCL8 in Colorectal Cancer. FRONT ONCOL. 2022; 12: 894043Read more>>
- Feng Huang. et al. NMR-based metabolomic analysis for the effects of moxibustion on imiquimod-induced psoriatic mice. J ETHNOPHARMACOL. 2023 Jan;300:115626Read more>>
- Wu, Bo. et al. Diagnostic values of plasma matrix metalloproteinase-7, interleukin-8, and gamma-glutamyl transferase in biliary atresia. EUR J PEDIATR. 2022 Sep;:1-9Read more>>
- Yuliani, Fara Silvia. et al. Thrombin induces IL-8/CXCL8 expression by DCLK1-dependent RhoA and YAP activation in human lung epithelial cells. J BIOMED SCI. 2022 Dec;29(1):1-22Read more>>
- Yu Xia. et al. Ferric citrate-induced colonic mucosal damage associated with oxidative stress, inflammation responses, apoptosis, and the changes of gut microbial composition. ECOTOX ENVIRON SAFE. 2023 Jan;249:114364Read more>>
- Ren, Xiaoli. et al. Expression and significance of IL-6 and IL-8 in canine mammary gland tumors. SCI REP-UK. 2023 Jan;13(1):1-9Read more>>
- Pengyuan Kang. et al. Interleukin 8 in plasma is an efficacy marker for advanced non-small cell lung cancer treated with hypofractionated radiotherapy and PD-1 blockade. CYTOKINE. 2023 Mar;163:156133Read more>>
- Chen Fanghua. et al. SSH1 promotes progression of intrahepatic cholangiocarcinoma via p38 MAPK-CXCL8 axis. CARCINOGENESIS. 2023 MaRead more>>
- Xingliang Dai. et al. Crosstalk between microglia and neural stem cells influences the relapse of glioblastoma in GBM immunological microenvironment. CLIN IMMUNOL. 2023 Jun;251:109333Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rabbit carotid artery labeled with Anti-IL-8/CXCL8 Polyclonal Antibody, Unconjugated (bs-0780R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (IL-8 ) Polyclonal Antibody, Unconjugated (bs-0780R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (IL-8 ) Polyclonal Antibody, Unconjugated (bs-0780R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (IL-8 ) Polyclonal Antibody, Unconjugated (bs-0780R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (IL-8 ) Polyclonal Antibody, Unconjugated (bs-0780R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (IL-8 ) Polyclonal Antibody, Unconjugated (bs-0780R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human esophagus carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (IL-8 ) Polyclonal Antibody, Unconjugated (bs-0780R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.