bs-0805R [Primary Antibody]
CD56 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD56

Immunogen Range: 621-720/858


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4684

Swiss Prot: P13591

Source: KLH conjugated synthetic peptide derived from human CD56

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 92


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Chicken
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sahiner et al. Impact of intravesical hyaluronic acid treatment on bladder inflammation in interstitial cystitis rat model. (2018) Int.Braz.J.Urol. 44:1014-1022Read more>>
  • Wu TH et al. The Combination of Astragalus membranaceus and Angelica sinensis Inhibits Lung Cancer and Cachexia through Its Immunomodulatory Function. J Oncol. 2019 Nov 3;2019:9206951. Read more>>
  • Yao Lei. et al. Phytochemical natural killer cells reprogram tumor microenvironment for potent immunotherapy of solid tumors. BIOMATERIALS. 2022 Jun;:121635Read more>>
  • Yuji Hayashi. et al. Simultaneous disturbance of NHE1 and LOXL2 decreases tumorigenicity of head and neck squamous cell carcinoma. AURIS NASUS LARYNX. 2024 Jun;51:472Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human esophageal carcinoma labeled with Rabbit Anti CD56/NCAM1 Polyclonal Antibody, Unconjugated (bs-0805R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti CD56/NCAM1 Polyclonal Antibody, Unconjugated (bs-0805R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse cerebral cortex lysates probed with CD56 Polyclonal Antibody, Unconjugated (bs-0805R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Tissue/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (CD56) polyclonal Antibody, Unconjugated (bs-0805R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Jurkat cells probed with CD56 Polyclonal Antibody, unconjugated (bs-0805R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)