bs-0806R-A750 [Conjugated Primary Antibody]
Collagen 4 Polyclonal Antibody, ALEXA FLUOR® 750 Conjugated
www.biossusa.com
[email protected]
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DATASHEET

Host: Rabbit

Target Protein: Collagen 4

Specificity: This antibody has 79% cross reactivity with Collagen alpha-5(IV) chain.

Immunogen Range: 1401-1500/1669


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 12826

Swiss Prot: P02463

Source: KLH conjugated synthetic peptide derived from mouse Collagen alpha-1(IV) chain

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin (By similarity).

Conjugation: ALEXA FLUOR® 750

Excitation/ Emission: 749nm/775nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 165


Cross Reactive Species: Human
Mouse
Rat
Rabbit

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang et al. MiR-130a-3p attenuates activation and induces apoptosis of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis by directly targeting TGFBR1 and TGFBR2. (2017) Cell.Death.Dis. 8:e2792Read more>>
  • Tatomir A et al. RGC-32 regulates reactive astrocytosis and extracellular matrix deposition in experimental autoimmune encephalomyelitis.Immunol Res. 2018 Jul 13.Read more>>
  • S Q et al. Cyanidin-3-glucoside from black rice prevents renal dysfunction and renal fibrosis in streptozotocin-diabetic rats. Journal of Functional Foods,(2020)72, 104062.Read more>>
  • Meng-Qi Tong. et al. Glucose-responsive hydrogel enhances the preventive effect of insulin and liraglutide on diabetic nephropathy of rats. Acta Biomater. 2021 Mar;122:111Read more>>
  • Su Yeon Oh. et al. Aesculetin Attenuates Alveolar Injury and Fibrosis Induced by Close Contact of Alveolar Epithelial Cells with Blood-Derived Macrophages via IL-8 Signaling. Int J Mol Sci. 2020 Jan;21(15):5518Read more>>
  • Su-Yeon Oh. et al. Aesculetin Inhibits Airway Thickening and Mucus Overproduction Induced by Urban Particulate Matter through Blocking Inflammation and Oxidative Stress Involving TLR4 and EGFR. Antioxidants-Basel. 2021 Mar;10(3):494Read more>>
  • Wang Hua. et al. Distribution of extracellular matrix related proteins in normal and cryptorchid ziwuling black goat testes. ANIM REPROD. 2022 Jun;19Read more>>
  • Guobao Chen. et al. In Situ Regulation and Mechanisms of 3D Matrix Stiffness on the Activation and Reversion of Hepatic Stellate Cells. ADV HEALTHC MATER. 2022 Dec;:220256Read more>>
  • Chengshan JIN. et al. Qianjin Wenwu decoction suppresses renal interstitial fibrosis by enhancing the degradation of extracellular matrix in mice with unilateral ureteral obstruction. CHIN J NAT MEDICINES. 2023 Apr;21:253Read more>>
VALIDATION IMAGES