Host: Rabbit
Target Protein: Fibulin 5
Immunogen Range: 101-200/448
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 10516
Swiss Prot: Q9UBX5
Source: KLH conjugated synthetic peptide derived from human Fibulin 5
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
Predicted Molecular Weight: 48
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species: Cow
For research use only. Not intended for diagnostic or therapeutic use.
- Liao, Yiwei, et al. "Fibulin-5 inhibits the cell proliferation, migration and angiogenesis in glioma." Int J Clin Exp Pathol 9.9 (2016): 8943-8952.Read more>>
L1 rat brain, L2 human colon carcinoma lysates probed (bs-0810R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 50kDa.
Paraformaldehyde-fixed, paraffin embedded Rat ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Fibulin 5 Polyclonal Antibody, Unconjugated (bs-0810R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse kidney lysates; Lane 2: Mouse large intestine lysates; probed with Fibulin 5 Polyclonal Antibody, unconjugated (bs-0810R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Lovo cell lysates probed with Fibulin 5 Polyclonal Antibody, Unconjugated (bs-0810R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.