bs-0813R-HRP [Conjugated Primary Antibody]
Casein Polyclonal Antibody, HRP Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Casein

Clonality: Polyclonal

Isotype: IgG

Source: Casein protein from bovine milk

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles

Background:

Casein is the name for a family of related phosphoproteins. These proteins are commonly found in mammalian milk, making up 80% of the proteins in cow milk and between 20% and 45% of the proteins in human milk. Casein has a wide variety of uses, from being a major component of cheese, to use as a food additive, to a binder for safety matches. As a food source, casein supplies amino acids; carbohydrates; and two inorganic elements, calcium and phosphorus. Casein from bovine milk is a phosphoprotein. There are four main types of casein which make up approximately 80% of the total protein in bovine milk: _-s1 casein, _-s2 casein, _-casein, and _-casein. Casein is proposed to be the main protective constituent in milk. 

Conjugation: HRP

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-F(1:100-500)

Predicted Molecular Weight: 23


Cross Reactive Species: Human
Mouse
Bovine
Others

Predicted Cross Reactive Species: Cow

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang, Boce, Ariella Feldman, and Qin Wang. "A novel insight in rapid allergen detection in food systems: From threshold dose to real-world concentration."Sensors and Actuators B: Chemical?186 (2013): 597-602.Read more>>
  • Blaauw, Brett R., Vincent P. Jones, and Anne L. Nielsen. "Utilizing immunomarking techniques to track Halyomorpha halys (Hemiptera: Pentatomidae) movement and distribution within a peach orchard." PeerJ 4 (2016): e1997.Read more>>
  • Blaauw, Brett R., et al. "Measuring host plant selection and retention of Halyomorpha halys by a trap crop." Entomologia Experimentalis et Applicata.Read more>>
  • Zhang, Minghui, et al. "Annexin A2 Positively Regulates Milk Synthesis and Proliferation of Bovine Mammary Epithelial Cells through the mTOR Signaling Pathway." Journal of Cellular Physiology (2017).Read more>>
  • Zhen, Zhen, et al. "DEAD-box helicase 6 (DDX6) is a new negative regulator for milk synthesis and proliferation of bovine mammary epithelial cells." In Vitro Cellular & Developmental Biology-Animal (2017): 1-9.Read more>>
  • Zhang S et al. The phosphorylation of Tudor-SN mediated by JNK is involved in the regulation of milk protein synthesis induced by prolactin in BMECs. (2018) J. Cell. Physiol. Sep 06Read more>>
  • Qi H et al. Methionine Promotes Milk Protein and Fat Synthesis and Cell Proliferation via the SNAT2-PI3K Signaling Pathway in Bovine Mammary Epithelial Cells.(2018) J Agric Food Chem.66(42):11027-11033.Read more>>
  • Huo N et al. PURB is a positive regulator of amino acid‐induced milk synthesis in bovine mammary epithelial cells.(2018) J Cell Physiol. Oct 26. Read more>>
  • Wang M et al. Protein 14-3-3ε Regulates Cell Proliferation and Casein Synthesis via PI3K-mTOR Pathway in Dairy Cow Mammary Epithelial Cells.(2018) J. Agric. Food Chem. Nov 14;66(45):12000-12008. Read more>>
  • Li B et al. Septin6 regulates cell growth and casein synthesis in dairy cow mammary epithelial cells viamTORC1 pathway. J Dairy Res. 2019 May;86(2):181-187. Read more>>
  • Yu M et al. Taurine promotes milk synthesis via the GPR87-PI3K-SETD1A signaling in BMECs. J Agric Food Chem. 2019 Feb 20;67(7):1927-1936. Read more>>
  • Dai W et al. ASCT2 is involved in SARS-mediated β-casein synthesis of bovine mammary epithelial cells with methionine supply. J Agric Food Chem. 2019 Oct 22.Read more>>
  • Luo C et al. Methionine stimulates GlyRS phosphorylation via the GPR87-CDC42/Rac1-MAP3K10 signaling pathway. Biochem Biophys Res Commun. 2020 Mar 19;523(4):847-852.Read more>>
  • Yang Yu et al. Vaccarin promotes proliferation of and milk synthesis in bovine mammary epithelial cells through the Prl receptor-PI3K signaling pathway. Eur J Pharmacol. 2020 Aug 5;880:173190.Read more>>
  • Xiaohan Yuanet al. NCOA5 is a master regulator of amino acid-induced mTOR activation and -casein synthesis in bovine mammary epithelial cells. Biochem Biophys Res Commun. 2020 Aug 27;529(3):569-574.Read more>>
  • Chaoxiang Chen. et al. General and mild modification of food-derived extracellular vesicles for enhanced cell targeting. Nanoscale. 2021 Feb;13(5):3061-3069Read more>>
  • Mengmeng Yu. et al. Daidzein promotes milk synthesis and proliferation of mammary epithelial cells via the estrogen receptor -dependent NFB1 activation. 2020 May 13Read more>>
  • Chaoxiang Chen. et al. Active cargo loading into extracellular vesicles: Highlights the heterogeneous encapsulation behaviour. J Extracell Vesicles. 2021 Nov;10(13):e12163Read more>>
  • Lampasona Timothy. et al. Behavioral Effects and Retention of Protein Immunomarkers on Plum Curculio Conotrachelus nenuphar (Coleoptera: Curculionidae). J Insect Sci. 2021 Nov;21(6)Read more>>
  • Lulu Wang. et al. METTL3 is a key regulator of milk synthesis in mammary epithelial cells. 2021 Dec 11Read more>>
VALIDATION IMAGES