bs-0817R [Primary Antibody]
MAGE-1 Polyclonal Antibody
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DATASHEET

Host: Rabbit

Target Protein: MAGE-1

Immunogen Range: 101-200/309


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4100

Swiss Prot: P43355

Source: KLH conjugated synthetic peptide derived from human MAGE-1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 34


Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Longfei Xiao. et al. Dihydrotestosterone through blockade of TGF-/Smad signaling mediates the anti-fibrosis effect under hypoxia in canine Sertoli cells. J Steroid Biochem. 2022 Feb;216:106041Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat lung melanoma labeled with Anti-MAGE-1 Polyclonal Antibody, Unconjugated (bs-0817R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MAGE-1 Polyclonal Antibody, Unconjugated (bs-0817R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.