bs-0829R [Primary Antibody]
Nanog Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Nanog

Immunogen Range: 71-170/305


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 71950

Swiss Prot: Q80Z64

Source: KLH conjugated synthetic peptide derived from mouse Nanog

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 34


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu, Yu-Hong, et al. "A signature for induced pluripotent stem cell–associated genes in colorectal cancer." Medical Oncology 30.1 (2013): 1-11.Read more>>
  • El-Badawy, Ahmed, et al. "Ovarian Extract Promotes Proliferation and Upregulates Pluripotency of Mesenchymal Stromal Cells." Journal of Ecology of Health & Environment (2016).Read more>>
  • El-Badawy, Ahmed, et al. "Cancer cell-soluble factors reprogram mesenchymal stromal cells to slow cycling, chemoresistant cells with a more stem-like state." Stem Cell Research & Therapy 8.1 (2017): 254.Read more>>
  • Liu et al. JNK is required for maintaining the tumor-initiating cell-like properties of acquired chemoresistant human cancer cells. (2015) Acta.Pharmacol.Si. 36:1099-106Read more>>
  • El-Badawy et al. Cancer cell-soluble factors reprogram mesenchymal stromal cells to slow cycling, chemoresistant cells with a more stem-like state. (2017) Stem.Cell.Res.Ther. 8:254Read more>>
  • Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/5 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93.Read more>>
  • Li X et al. A comparative study of the behavior of neural progenitor cells in extrusion-based in vitro hydrogel models. Biomed Mater. 2019 Sep 9;14(6):065001. Read more>>
  • Kim CW et al. Effects of cigarette smoke components on myocardial differentiation of mouse embryonic stem cells. Environ Toxicol. 2019 Sep 10.Read more>>
  • Wu Q et al. Expression pattern Nanog is associated with the development of gestational trophoblastic neoplasia in Chinese women from Fujian province: a case-control study. Gynecol Endocrinol. 2020 Jun 25;1-5.Read more>>
  • Sung-Moo Lee. et al. Effect of the phenylpyrrole fungicide fludioxonil on cell proliferation and cardiac differentiation in mouse embryonic stem cells. Reprod Toxicol. 2021 Sep;104:76Read more>>
  • Lu, Yinglin. et al. Ascorbic acid and all-trans retinoic acid promote proliferation of chicken blastoderm cells (cBCs) by mediating DNA demethylation. In Vitro Cell Dev-An. 2022 Mar;:1-11Read more>>
VALIDATION IMAGES

Lane 1: A549 cell lysates probed with Rabbit Anti-Nanog Polyclonal Antibody, Unconjugated (bs-0829R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.


Formalin-fixed and paraffin embedded mouse tooth germ tissue labeled with Anti-Nanog Polyclonal Antibody (bs-0829R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining


HeLa cells probed with Estrogen Receptor alpha + beta Antibody, unconjugated (bs-0829R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)