bs-0832R [Primary Antibody]
MICA Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MICA

Immunogen Range: 120-170/318


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 100507436

Swiss Prot: Q29983

Source: KLH conjugated synthetic peptide derived from human MICA

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 43


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen, Han, et al. "Scorpion venom activates natural killer cells in hepatocellular carcinoma via the NKG2D-MICA pathway." International Immunopharmacology 35 (2016): 307-314.Read more>>
  • Cheney, Ian Wayne. "METHODS AND DEVICES FOR REMOVAL OF IMMUNOSUPPRESSIVE LIGANDS." U.S. Patent No. 20,160,030,659. 4 Feb. 2016.Read more>>
  • Jie Wang. et al. BDNF-overexpressing human umbilical cord mesenchymal stem cell-derived motor neurons improve motor function and prolong survival in amyotrophic lateral sclerosis mice. Neurol Res. 2021;43(3):199-209Read more>>
  • Yao Lei. et al. Phytochemical natural killer cells reprogram tumor microenvironment for potent immunotherapy of solid tumors. BIOMATERIALS. 2022 Jun;:121635Read more>>
  • Xu, Xi. et al. PD-1 signalling defines and protects leukaemic stem cells from T?cell?receptor-induced cell death in T?cell acute lymphoblastic leukaemia. NAT CELL BIOL. 2023 Jan;:1-13Read more>>
  • Xicai Li. et al. Inhibition of Checkpoint Kinase 1 (CHK1) Upregulates Interferon Regulatory Factor 1 (IRF1) to Promote Apoptosis and Activate Anti-Tumor Immunity via MICA in Hepatocellular Carcinoma (HCC). CANCERS. 2023 Jan;15(3):85Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-MICA/MHC I a Polyclonal Antibody, Unconjugated (bs-0832R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-MICA/MHC I a Polyclonal Antibody, Unconjugated (bs-0832R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded mouse lung labeled with Anti-MICA/MHC I a Polyclonal Antibody, Unconjugated (bs-0832R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Lane 1: Mouse Large intestine tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Mouse Liver tissue lysates probed with MICA Polyclonal Antibody, Unconjugated (bs-0832R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.