bs-0849R [Primary Antibody]
APOA1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: APOA1

Immunogen Range: 51-150/267


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 335

Swiss Prot: P02647

Source: KLH conjugated synthetic peptide derived from human APOA1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 28


Cross Reactive Species: Human
Mouse
Rat
Chicken

Predicted Cross Reactive Species: Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ma P et al. Mass spectrometric analysis of active ingredients in fertilized egg for lipid metabolism. 29 January 2020, PREPRINT (Version 1) available at Research Square.Read more>>
  • Liu, Qian, et al. "Proteomic Study on Usnic Acid-induced Hepatotoxicity in Rats." Journal of agricultural and food chemistry (2012).Read more>>
  • Wu, Chun-Yan, et al. "Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers." Proteome Science 14.1 (2016): 9.Read more>>
  • Zhang, Pengfei, et al. "Proteome analysis of egg yolk after exposure to zinc oxide nanoparticles." Theriogenology (2017).Read more>>
  • Wu et al. Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers. (2016) Proteome.Sc. 14:9Read more>>
  • "Ma P et al. Mass spectrometric analysis of active ingredients in fertilized egg for lipid metabolism. 29 January 2020, PREPRINT (Version 1) available at Research Square."Read more>>
  • Ling Yang. et al. Effect of functional single nucleotide polymorphism g.-572 A?>?G of apolipoprotein A1 gene on resistance to ketosis in Chinese Holstein cows. Res Vet Sci. 2020 OcRead more>>
  • ingxiang Wang. et al. Atherosclerosis-associated hepatic secretion of VLDL but not PCSK9 is dependent on cargo receptor protein Surf4. J Lipid Res. 2021 Jun;:100091Read more>>
  • Xuting Liu. et al. Serum apolipoprotein A-I depletion is causative to silica nanoparticlesCinduced cardiovascular damage. P Natl Acad Sci Usa. 2021 Nov;118(44)Read more>>
  • Li Xu. et al. Yinchenhao Tang alleviates high fat diet induced NAFLD by increasing NR1H4 and APOA1 expression. Journal of Traditional and Complementary Medicine. 2023 FebRead more>>
  • Kai Chen. et al. Cerebrospinal Fluid Proteomic Profiles in Patients with Postherpetic Neuralgia. J PROTEOME RES. 2023;XXXX(XXX):XXX-XXXRead more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-APOA1 Polyclonal Antibody, Unconjugated (bs-0849R) at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining.


Mouse liver lysates probed with Anti-APOA1 Polyclonal Antibody, Unconjugated (bs-0849R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C