bs-0862R [Primary Antibody]
Insulin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Insulin

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 397415

Swiss Prot: P01315

Source: KLH conjugated Insulin from porcine pancreas

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
IP(1-2ug)

Predicted Molecular Weight: 3-Dec


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xue, J. L., et al. "Distribution and Role of Receptor Interaction Protein 140 in Pancreatic β-Cells In Rodents, in vivo And in vitro." Acta Endocrinologica (Buc) 10.1 (2014): 41-52.Read more>>
  • Zhao, Kai, et al. "Preparation, Characterization and Hypoglycaemic Effects of Orally Delivered Insulin-Loaded PLGA Nanoparticles in Diabetic Rats." Science of Advanced Materials 7.6 (2015): 1114-1124.Read more>>
  • Sandikci, M. et al. “Changes on the Pancreas in Experimental Diabetes and the Effect of Lycopene on These Changes: Pdx‐1, Ngn‐3, and Nestin Expressions.” The Anatomical Record (2017)Read more>>
  • Xu X et al. Potential biomarker of fibroblast growth factor 21 in valproic acid-treated livers. Biofactors. 2019 May 23. Read more>>
  • Vlahos AE et al. Endothelialized collagen based pseudo-islets enables tuneable subcutaneous diabetes therapy. Biomaterials. 2019 Dec 26;232:119710. Read more>>
  • Wang J et al. Effect of Asthma on Erectile Dysfunction in Rats as Determined by Biological Network AnalysisMed Sci Monit.2020 Dec 20;26:e927491.Read more>>
  • Hsiao-Pei Tsai. et al. Risperidone Exacerbates Glucose Intolerance, Nonalcoholic Fatty Liver Disease, and Renal Impairment in Obese Mice. Int J Mol Sci. 2021 Jan;22(1):409Read more>>
  • Ji-sheng Wang. et al. Study on the Mechanism of Jiawei Shengjiang Powder in Improving Male Asthma-Induced Asthenospermia Based on Network Pharmacology and Bioinformatics. Drug Des Dev Ther. 2021 Mar;15:1245-1259Read more>>
  • Yuhua Gao. et al. Pancreatic Duct Cells Isolated From Canines Differentiate Into Beta-Like Pancreatic Islet Cells. Front Vet Sci. 2021; 8: 771196Read more>>
  • Hany Ezzat Khalil. et al. Mechanistic Insights into the Ameliorative Effect of Cichoriin on Diabetic Rats—Assisted with an In Silico Approach. MOLECULES. 2022 Jan;27(21):7192Read more>>
VALIDATION IMAGES

Lane 1: human islet alpha lysates Lane 2: human islet beta lysates probed with Rabbit Anti-Insulin Polyclonal Antibody, Unconjugated (bs-0862R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.