bs-0876R [Primary Antibody]
AKT1/2/3 (Ser472/Ser473/Ser474) Antibody
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]

Host: Rabbit

Target Protein: AKT1/2/3 (Ser472/Ser473/Ser474)

Modification Site: Ser473

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24185

Swiss Prot: P47196

Source: KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..


AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)

Predicted Molecular Weight: 56

Cross Reactive Species: Human

Predicted Cross Reactive Species: Dog

For research use only. Not intended for diagnostic or therapeutic use.

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Formalin-fixed and paraffin embedded human endometrium tissue labeled with Anti-phospho-AKT/PKB(Ser473) Polyclonal Antibody, Unconjugated (bs-0876R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse tumor with labeled Anti-phospho-AKT/PKB(Ser473) Polyclonal Antibody, Unconjugated (bs-0876R) at 1:600, followed by conjugation to the secondary antibody and DAB staining

U937 cell lysates probed with Anti-AKT1/2/3 (Ser472/Ser473/Ser474) Polyclonal Antibody, Unconjugated (bs-0876R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Mouse liver lysates probed with Rabbit Anti-AKT(Ser473) Polyclonal Antibody, Unconjugated (bs-0876) at 1:300 overnight at 4˚C. Incubate with HRP conjugated Goat-Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000 for 90min at 37˚C.