VALIDATION IMAGES
Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-CD144/VECD/VE Cadherin Polyclonal Antibody, Unconjugated (bs-0878R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
A549 lyates probed with Rabbit Anti-VE Cadherin Polyclonal Antibody, Unconjugated (bs-0878R) at 1:300 in 4˚C overnight. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.
Image provided by One World Lab validation program. HEK293 cells probed with Rabbit Anti-VE Cadherin Polyclonal Antibody (bs-0878R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-VE Cadherin Polyclonal Antibody (bs-0878R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Mouse heart lysates probed with VE Cadherin Polyclonal Antibody, unconjugated (bs-0878R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Images provided by the Independent Validation Program, badge number 029590:Formalin-fixed and frozen-OCT embedded mouse liver labeled with Rabbit Anti-VE Cadherin Polyclonal Antibody, Unconjugated(bs-0878R) 1:100, overnight at 4°C, The secondary antibody was diluted to 1:100 for 120 minutes at 37°C.
Mouse kidney lysates probed with VE Cadherin Polyclonal Antibody, Unconjugated (bs-0878R) at 3ug for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).