bs-0891R [Primary Antibody]
Pokemon Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Pokemon

Immunogen Range: 151-250/569


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 51341

Swiss Prot: O95365

Source: KLH conjugated synthetic peptide derived from human Pokemon

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity). Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 63


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhao, Yi, et al. "Pokemon enhances proliferation, cell cycle progression and anti-apoptosis activity of colorectal cancer independently of p14ARF–MDM2–p53 pathway." Medical Oncology 31.12 (2014): 1-8.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colon carcinoma with Anti-Pokemon Polyclonal Antibody, Unconjugated (bs-0891R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human gastric carcinoma with Anti-Pokemon Polyclonal Antibody, Unconjugated (bs-0891R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


293T lysates probed with PokemonZBTB7A Polyclonal Antibody, Unconjugated (bs-0891R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.