bs-0913R [Primary Antibody]
Mtp53 (N235K N239Y) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Mtp53 N235K N239Y

Immunogen Range: 230-241/393


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7157

Swiss Prot: P04637

Source: KLH conjugated synthetic peptide derived from human Mtp53 around N235K N239Y

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 21


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Huang, Yanhong, et al. "Differential expression patterns and clinical significance of estrogen receptor-alpha and beta in papillary thyroid carcinoma." BMC Cancer 14.1 (2014): 383.Read more>>
  • Zhao, Yi, et al. "Pokemon enhances proliferation, cell cycle progression and anti-apoptosis activity of colorectal cancer independently of p14ARF–MDM2–p53 pathway." Medical Oncology 31.12 (2014): 1-8.Read more>>
  • Dong et al. Altered expression of estrogen receptor β2 is associated with different biological markers and clinicopathological factors in papillary thyroid cancer. (2015) Int.J.Clin.Exp.Pathol. 8:7149-56Read more>>
  • Okoye JO et al. Mutant p53 protein is prevalent in Aflatoxin B1 than in hepatitis B core antigen associated chronic liver diseases in Southern Nigeria. JOURNAL OF INTERDISCIPLINARY HISTOPATHOLOGY, 2019,7(1):10–19.Read more>>
VALIDATION IMAGES

MCF-7 cells probed with Mtp53 (N235K N239Y) Polyclonal Antibody, unconjugated (bs-0913R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)