bs-0921R [Primary Antibody]
SIRT1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SIRT1

Immunogen Range: 101-200/747


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 23411

Swiss Prot: Q96EB6

Source: KLH conjugated synthetic peptide derived from human SIRT1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 58/81


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Mohan, Mahesh, et al. "Dysregulated miR34a SIRT1 Acetyl p65 Axis Is a Potential Mediator of Immune Activation in the Colon during Chronic Simian Immunodeficiency Virus Infection of Rhesus Macaques." The Journal of Immunology (2014): 1401447.Read more>>
  • Yan, Dongying, et al. "Effects of Aluminium on long‐term Memory in Rats and on SIRT1 Mediating the Transcription of CREB‐Dependent Gene in Hippocampus." Basic & Clinical Pharmacology & Toxicology (2017).Read more>>
  • Hu, Zheng, et al. "5-Aminolevulinic acid-mediated sonodynamic therapy induces anti-tumor effects in malignant melanoma via p53-miR-34a-Sirt1 axis." Journal of dermatological science 79.2 (2015): 155-162.Read more>>
  • Shi et al. Vam3, a derivative of resveratrol, attenuates cigarette smoke-induced autophagy. (2012) Acta.Pharmacol.Si. 33:888-96Read more>>
  • Zeng et al. Hypothermic machine perfusion ameliorates inflammation during ischemia‑reperfusion injury via sirtuin‑1‑mediated deacetylation of nuclear factor‑κB p65 in rat livers donated after circulatory death. (2017) Mol.Med.Rep. 16:8649-8656Read more>>
  • Gao D et al. Protective effect of astaxanthin against contrast-induced acute kidney injury via SIRT1-p53 pathway in rats.(2018)Int Urol NephrolRead more>>
  • Chen H et al. Sevoflurane attenuates cognitive dysfunction and NLRP3-dependent caspase-1/11-GSDMD pathway-mediated pyroptosis in the hippocampus via upregulation of SIRT1 in a sepsis model. Arch Physiol Biochem. 2020 Jun 13;1-8.Read more>>
  • Kai Kanget al. FGF21 attenuates neurodegeneration through modulating neuroinflammation and oxidant-stress. Biomed Pharmacother. 2020 Sep;129:110439.Read more>>
  • Ming Qiet al. Postnatal growth retardation is associated with intestinal mucosa mitochondrial dysfunction and aberrant energy status in piglets. J Cell Mol Med. 2020 Sep;24(17):10100-10111.Read more>>
  • Qi Guo. et al. Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via APPL1CSIRT1CPGC-1. Diabetologia. 2020 Dec;63(12):2675-2688Read more>>
  • Shi Lina. et al. Sirt1 Regulates Oxidative Stress in Oxygen-Glucose Deprived Hippocampal Neurons. Front Pediatr. 2020 Aug;8:455Read more>>
  • Haichao Lin. et al. HMGR overexpression and interference affect the expression of steroidogenic genes and cholesterol content in bovine intramuscular adipocytes. Sci Rep-Uk. 2020 Oct;10(1):1-8Read more>>
  • Xu Sun. et al. FGF21 Enhances Therapeutic Efficacy and Reduces Side Effects of Dexamethasone in Treatment of Rheumatoid Arthritis. Inflammation. 2021 Feb;44(1):249-26Read more>>
  • iqi Han. et al. Harmful Effects of Inorganic Mercury Exposure on Kidney Cells: Mitochondrial Dynamics Disorder and Excessive Oxidative Stress. 2021 May 31Read more>>
  • Da HU. et al. Nicotinic Acid against Acetaminophen-Induced Hepatotoxicity via Sirt1/Nrf2 Antioxidative Pathway in Mice. J Nutr Sci Vitaminol. 2021 Jun;67(3):145-152Read more>>
  • Hyo Won Jung. et al. Anticancer Effects of Ursi Fel Extract and Its Active Compound, Ursodeoxycholic Acid, in FRO Anaplastic Thyroid Cancer Cells. Molecules. 2021 Jan;26(17):5309Read more>>
  • Lin B. et al. Antioxidant Effects of <i>Sophora davidi</i> (Franch.) Skeels on d-Galactose-Induced Aging Model in Mice <i>via</i> Activating the SIRT1/p53 Pathway.. Front Pharmacol. 2021 Dec;12:754554-754554Read more>>
  • Wangrui Lei. et al. Psoralidin protects against cerebral hypoxia/reoxygenation injury: Role of GAS6/Axl signaling. PHYTOTHER RES. 2022 May 18Read more>>
  • Guangning Kou. et al. Sesamin Activates Skeletal Muscle FNDC5 Expression and Increases Irisin Secretion via the SIRT1 Signaling Pathway. J AGR FOOD CHEM. 2022;XXXX(XXX):XXX-XXXRead more>>
  • Zhihui Liu. et al. Curcumin alleviates aristolochic acid nephropathy based on SIRT1/Nrf2/HO-1 signaling pathway. TOXICOLOGY. 2022 Sep;479:153297Read more>>
  • in Li. et al. Corilagin alleviates intestinal ischemia/reperfusion injury by relieving oxidative stress and apoptosis via AMPK/Sirt1-autophagy pathway. EXP BIOL MED. ;()Read more>>
  • Yue Shen. et al. Lycopene prevents Di-(2-ethylhexyl) phthalate-induced mitophagy and oxidative stress in mice heart via modulating mitochondrial homeostasis. J NUTR BIOCHEM. 2023 May;115:109285Read more>>
  • Jinjing Jia. et al. Thioredoxin-1 Promotes Mitochondrial Biogenesis Through Regulating AMPK/Sirt1/PGC1 Pathway in Alzheimer's Disease. ASN NEURO. ;()Read more>>
  • Ruonan Wang. et al. The role of mitochondrial dynamics imbalance in hexavalent chromium-induced apoptosis and autophagy in rat testis. CHEM-BIOL INTERACT. 2023 Apr;374:110424Read more>>
  • Gizem Usluel. et al. The effects of Spirulina platensis on behavior in adolescent rats fed a high-fat diet. INT J DEV NEUROSCI. 2023 JuRead more>>
  • Yu Xia. et al. Astilbin targeted Sirt1 to inhibit acetylation of Nrf2 to alleviate grass carp hepatocyte apoptosis caused by PCB126-induced mitochondrial kinetic and metabolism dysfunctions. FISH SHELLFISH IMMUN. 2023 Oct;141:109Read more>>
  • Weiping Li. et al. Silibinin exerts neuroprotective effects against cerebral hypoxia/reoxygenation injury by activating the GAS6/Axl pathway. TOXICOLOGY. 2023 Aug;:153598Read more>>
  • Dae-Hyun Lee. et al. Increased IGFBP2 Levels by Placenta-Derived Mesenchymal Stem Cells Enhance Glucose Metabolism in a TAA-Injured Rat Model via AMPK Signaling Pathway. INT J MOL SCI. 2023 Jan;24(22):16531Read more>>
  • Jianhui Liu. et al. Melatonin ameliorates PM2.5-induced spermatogenesis disorder by preserving H3K9 methylation and SIRT3. ENVIRON TOXICOL. 2023 NovRead more>>
VALIDATION IMAGES

Hela cell lysates probed with Anti-SIRT1 Polyclonal Antibody, Unconjugated (bs-0921R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C.


Protein: mouse heart lysate; Primary: rabbit Anti-SIRT1 (bs-0921R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 58/81 kDa Observed band size: 58 kDa


Formalin-fixed and paraffin embedded human endometrial carcinoma labeled with Anti-SIRT1 Polyclonal Antibody, Unconjugated (bs-0921R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-SIRT1 Polyclonal Antibody, Unconjugated (bs-0921R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


MCF-7 cells probed with SIRT1 Polyclonal Antibody, unconjugated (bs-0921R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)


HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SIRT1 Polyclonal Antibody(bs-0921R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SIRT1Polyclonal Antibody(bs-0921R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SIRT1 Polyclonal Antibody, Unconjugated (bs-0921R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SIRT1 Polyclonal Antibody, Unconjugated (bs-0921R) at 1:200 overnight at 4°C, DAB staining.