bs-0921R [Primary Antibody]
SIRT1 Polyclonal Antibody
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Host: Rabbit

Target Protein: SIRT1

Immunogen Range: 101-200/747


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 23411

Swiss Prot: Q96EB6

Source: KLH conjugated synthetic peptide derived from human SIRT1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:50-200)
ELISA(1:500-2000)
IHC-F(1:100-500)
IP(1:5000-10000)
IF(1:100-500)
IHC-P(1:100-500)

Predicted Molecular Weight: 58/81


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Mohan, Mahesh, et al. "Dysregulated miR34a SIRT1 Acetyl p65 Axis Is a Potential Mediator of Immune Activation in the Colon during Chronic Simian Immunodeficiency Virus Infection of Rhesus Macaques." The Journal of Immunology (2014): 1401447.Read more>>
  • Yan, Dongying, et al. "Effects of Aluminium on long‐term Memory in Rats and on SIRT1 Mediating the Transcription of CREB‐Dependent Gene in Hippocampus." Basic & Clinical Pharmacology & Toxicology (2017).Read more>>
  • Hu, Zheng, et al. "5-Aminolevulinic acid-mediated sonodynamic therapy induces anti-tumor effects in malignant melanoma via p53-miR-34a-Sirt1 axis." Journal of dermatological science 79.2 (2015): 155-162.Read more>>
  • Shi et al. Vam3, a derivative of resveratrol, attenuates cigarette smoke-induced autophagy. (2012) Acta.Pharmacol.Si. 33:888-96Read more>>
  • Zeng et al. Hypothermic machine perfusion ameliorates inflammation during ischemia‑reperfusion injury via sirtuin‑1‑mediated deacetylation of nuclear factor‑κB p65 in rat livers donated after circulatory death. (2017) Mol.Med.Rep. 16:8649-8656Read more>>
  • Gao D et al. Protective effect of astaxanthin against contrast-induced acute kidney injury via SIRT1-p53 pathway in rats.(2018)Int Urol NephrolRead more>>
  • Chen H et al. Sevoflurane attenuates cognitive dysfunction and NLRP3-dependent caspase-1/11-GSDMD pathway-mediated pyroptosis in the hippocampus via upregulation of SIRT1 in a sepsis model. Arch Physiol Biochem. 2020 Jun 13;1-8.Read more>>
  • Kai Kanget al. FGF21 attenuates neurodegeneration through modulating neuroinflammation and oxidant-stress. Biomed Pharmacother. 2020 Sep;129:110439.Read more>>
  • Ming Qiet al. Postnatal growth retardation is associated with intestinal mucosa mitochondrial dysfunction and aberrant energy status in piglets. J Cell Mol Med. 2020 Sep;24(17):10100-10111.Read more>>
  • Qi Guo. et al. Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via APPL1CSIRT1CPGC-1. Diabetologia. 2020 Dec;63(12):2675-2688Read more>>
  • Shi Lina. et al. Sirt1 Regulates Oxidative Stress in Oxygen-Glucose Deprived Hippocampal Neurons. Front Pediatr. 2020 Aug;8:455Read more>>
  • Haichao Lin. et al. HMGR overexpression and interference affect the expression of steroidogenic genes and cholesterol content in bovine intramuscular adipocytes. Sci Rep-Uk. 2020 Oct;10(1):1-8Read more>>
  • Xu Sun. et al. FGF21 Enhances Therapeutic Efficacy and Reduces Side Effects of Dexamethasone in Treatment of Rheumatoid Arthritis. Inflammation. 2021 Feb;44(1):249-26Read more>>
  • iqi Han. et al. Harmful Effects of Inorganic Mercury Exposure on Kidney Cells: Mitochondrial Dynamics Disorder and Excessive Oxidative Stress. 2021 May 31Read more>>
  • Da HU. et al. Nicotinic Acid against Acetaminophen-Induced Hepatotoxicity via Sirt1/Nrf2 Antioxidative Pathway in Mice. J Nutr Sci Vitaminol. 2021 Jun;67(3):145-152Read more>>
  • Hyo Won Jung. et al. Anticancer Effects of Ursi Fel Extract and Its Active Compound, Ursodeoxycholic Acid, in FRO Anaplastic Thyroid Cancer Cells. Molecules. 2021 Jan;26(17):5309Read more>>
  • Lin B. et al. Antioxidant Effects of <i>Sophora davidi</i> (Franch.) Skeels on d-Galactose-Induced Aging Model in Mice <i>via</i> Activating the SIRT1/p53 Pathway.. Front Pharmacol. 2021 Dec;12:754554-754554Read more>>
  • Wangrui Lei. et al. Psoralidin protects against cerebral hypoxia/reoxygenation injury: Role of GAS6/Axl signaling. PHYTOTHER RES. 2022 May 18Read more>>
  • Guangning Kou. et al. Sesamin Activates Skeletal Muscle FNDC5 Expression and Increases Irisin Secretion via the SIRT1 Signaling Pathway. J AGR FOOD CHEM. 2022;XXXX(XXX):XXX-XXXRead more>>
  • Zhihui Liu. et al. Curcumin alleviates aristolochic acid nephropathy based on SIRT1/Nrf2/HO-1 signaling pathway. TOXICOLOGY. 2022 Sep;479:153297Read more>>
  • in Li. et al. Corilagin alleviates intestinal ischemia/reperfusion injury by relieving oxidative stress and apoptosis via AMPK/Sirt1-autophagy pathway. EXP BIOL MED. ;()Read more>>
  • Yue Shen. et al. Lycopene prevents Di-(2-ethylhexyl) phthalate-induced mitophagy and oxidative stress in mice heart via modulating mitochondrial homeostasis. J NUTR BIOCHEM. 2023 May;115:109285Read more>>
  • Jinjing Jia. et al. Thioredoxin-1 Promotes Mitochondrial Biogenesis Through Regulating AMPK/Sirt1/PGC1 Pathway in Alzheimer's Disease. ASN NEURO. ;()Read more>>
  • Ruonan Wang. et al. The role of mitochondrial dynamics imbalance in hexavalent chromium-induced apoptosis and autophagy in rat testis. CHEM-BIOL INTERACT. 2023 Apr;374:110424Read more>>
  • Gizem Usluel. et al. The effects of Spirulina platensis on behavior in adolescent rats fed a high-fat diet. INT J DEV NEUROSCI. 2023 JuRead more>>
  • Yu Xia. et al. Astilbin targeted Sirt1 to inhibit acetylation of Nrf2 to alleviate grass carp hepatocyte apoptosis caused by PCB126-induced mitochondrial kinetic and metabolism dysfunctions. FISH SHELLFISH IMMUN. 2023 Oct;141:109Read more>>
  • Weiping Li. et al. Silibinin exerts neuroprotective effects against cerebral hypoxia/reoxygenation injury by activating the GAS6/Axl pathway. TOXICOLOGY. 2023 Aug;:153598Read more>>
  • Dae-Hyun Lee. et al. Increased IGFBP2 Levels by Placenta-Derived Mesenchymal Stem Cells Enhance Glucose Metabolism in a TAA-Injured Rat Model via AMPK Signaling Pathway. INT J MOL SCI. 2023 Jan;24(22):16531Read more>>
  • Jianhui Liu. et al. Melatonin ameliorates PM2.5-induced spermatogenesis disorder by preserving H3K9 methylation and SIRT3. ENVIRON TOXICOL. 2023 NovRead more>>
  • Agata Wroska. et al. High-Dose Fenofibrate Stimulates Multiple Cellular Stress Pathways in the Kidney of Old Rats. INT J MOL SCI. 2024 Jan;25(5):3038Read more>>
  • Zhao Xu. et al. Sirt1 inhibits macrophage polarization and inflammation in gouty arthritis by inhibiting the MAPK/NF-B/AP-1 pathway and activating the Nrf2/HO-1 pathway. INFLAMM RES. 2024 May;:1-12Read more>>
  • Li Zhipeng. et al. The role of quercetin in ameliorating bleomycin-induced pulmonary fibrosis: insights into autophagy and the SIRT1/AMPK signaling pathway. MOL BIOL REP. 2024 Dec;51(1):1-1Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human endometrial carcinoma labeled with Anti-SIRT1 Polyclonal Antibody, Unconjugated (bs-0921R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-SIRT1 Polyclonal Antibody, Unconjugated (bs-0921R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SIRT1 Polyclonal Antibody, Unconjugated (bs-0921R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SIRT1 Polyclonal Antibody, Unconjugated (bs-0921R) at 1:200 overnight at 4°C, DAB staining.


25 ug total protein per lane of various lysates (see on figure) probed with SIRT1 polyclonal antibody, unconjugated (bs-0921R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.