bs-0923R [Primary Antibody]
PDX1 Polyclonal Antibody
www.biossusa.com
[email protected]
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DATASHEET

Host: Rabbit

Target Protein: PDX1

Immunogen Range: 188-284/284


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 18609

Swiss Prot: P52946

Source: KLH conjugated synthetic peptide derived from mouse PDX1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds the DNA sequence 5'-CC[CT]TAATGGG-3'.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(IHC-P)(1:100-500)
IF(IHC-F)(1:100-500)
IF(ICC)(1:100-500)

Predicted Molecular Weight: 30


Cross Reactive Species: Rat
Goat

Predicted Cross Reactive Species: Human
Mouse
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Hefei, Wang, et al. "Morphological Characteristics and Identification of Islet‐like Cells Derived From Rat Adipose‐derived Stem Cells Cocultured with Pancreas Adult Stem Cells." Cell Biology International (2014).Read more>>
  • Jian, Ruo-Lei, et al. "Generation of insulin-producing cells from C3H10T1/2 mesenchymal progenitor cells." Gene (2015).Read more>>
  • Zheng, Liming, et al. "The Modification of Tet1 in Male Germline Stem Cells and Interact with PCNA, HDAC1 to promote their Self-renewal and Proliferation." Scientific Reports 6 (2016): 37414.Read more>>
  • Wang et al. Effect of Wnt Signaling on the Differentiation of Islet β-Cells from Adipose-Derived Stem Cells. (2017) Biomed.Res.In. 2017:2501578Read more>>
  • Sandikci, M. et al. “Changes on the Pancreas in Experimental Diabetes and the Effect of Lycopene on These Changes: Pdx‐1, Ngn‐3, and Nestin Expressions.” The Anatomical Record (2017)Read more>>
  • Chi-Hao Tsaiet al. The Powdered Root of Eurycoma longifolia Jack Improves Beta-Cell Number and Pancreatic Islet Performance through PDX1 Induction and Shows Antihyperglycemic Activity in db/db Mice. Nutrients. 2020 Jul 16;12(7):2111.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-PDX-1 Polyclonal Antibody, Unconjugated (bs-0923R) at 1:200, followed by conjugation to the secondary antibody and DAB staining