bs-0943R [Primary Antibody]
Androgen Receptor(Ser578) Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Androgen Receptor Ser578

Specificity: This phosphorylation site is homologous to that of Ser558 in Mouse and Ser561 in Rat.

Immunogen Range: 550-600/919


Modification Site: Ser578

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 367

Swiss Prot: P10275

Source: KLH conjugated synthetic phosphopeptide derived from human Androgen Receptor around the phosphorylation site of Ser578 [CG(p-S)CK]

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.

Size: 100ul

Concentration: 1ug/ul

Applications: IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 99


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti phospho-Androgen Receptor(Ser578) Polyclonal Antibody, Unconjugated (bs-0943R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded (mouse prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Androgen Receptor(Ser578)) Polyclonal Antibody, Unconjugated (bs-0943R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.