Host: Rabbit
Target Protein: ADRB2
Immunogen Range: 201-300/418
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 154
Swiss Prot: P07550
Source: KLH conjugated synthetic peptide derived from human of human ADRB2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 46
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Tibenska V et al. Gradual cold acclimation induces cardioprotection without affecting beta-adrenergic receptor-mediated adenylyl cyclase signaling. J Appl Physiol (1985). 2020 Apr 1;128(4):1023-1032.Read more>>
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- Revathikumar et al. Microsomal prostaglandin E synthase-1 gene deletion impairs neuro-immune circuitry of the cholinergic anti-inflammatory pathway in endotoxaemic mouse spleen. (2018) PLoS.One. 13:e0193210Read more>>
- Martín-Cordero L et al. β2 Adrenergic Regulation of the Phagocytic and Microbicide Capacity of Macrophages from Obese and Lean Mice: Effects of Exercise. Nutrients. 2019 Nov 9;11(11). pii: E2721. Read more>>
- Tibenska V et al. Gradual cold acclimation induces cardioprotection without affecting beta-adrenergic receptor-mediated adenylyl cyclase signaling. J Appl Physiol (1985). 2020 Apr 1;128(4):1023-1032.Read more>>
- Liu Z et al. Over-expression of microRNA-145 drives alterations in -adrenergic signaling and attenuates cardiac remodeling in heart failure post myocardial infarction. Aging (Albany NY). 2020 Jun 18;12(12):11603-11622.Read more>>
- Jinyao Liuet al. Alcohol consumption combined with dietary low-carbohydrate/high-protein intake increased the left ventricular systolic dysfunction risk and lethal ventricular arrhythmia susceptibility in apolipoprotein E/low-density lipoprotein receptor double-knockout mice. Alcohol. 2020 Dec;89:63-74.Read more>>
- Katarina Andelova. et al. Cardiac Cx43 Signaling Is Enhanced and TGF-β1/SMAD2/3 Suppressed in Response to Cold Acclimation and Modulated by Thyroid Status in Hairless SHRM. BIOMEDICINES. 2022 Jul;10(7):1707Read more>>
- Chuanju Zong. et al. Chronic restraint stress promotes gastric epithelial malignant transformation by activating the Akt/p53 signaling pathway via ADRB2. ONCOL LETT. 2022 Sep;24(3):1-11Read more>>
- Katsuaki Ieguchi. et al. The Sympathetic Nervous System Contributes to the Establishment of Pre-Metastatic Pulmonary Microenvironments. INT J MOL SCI. 2022 Jan;23(18):10652Read more>>
- Shuang Zhou. et al. Tumor microenvironment adrenergic nerves blockade liposomes for cancer therapy. J CONTROL RELEASE. 2022 Nov;351:656Read more>>
- Yunjung Lee. et al. Synergistic effect of n-3 PUFA and probiotic supplementation on bone loss induced by chronic mild stress through the brainCgutCbone axis. J FUNCT FOODS. 2023 Jan;100:105363Read more>>
- Lina S. Farhoumand. et al. Blockade of ß-Adrenergic Receptors by Nebivolol Enables Tumor Control Potential for Uveal Melanoma in 3D Tumor Spheroids and 2D Cultures. INT J MOL SCI. 2023 Jan;24(6):5894Read more>>
- Noam Ben-Shalom. et al. 2-Adrenergic Signaling Promotes Higher-Affinity B Cells and Antibodies. BRAIN BEHAV IMMUN. 2023 JuRead more>>
L1 rat brain lysates L2 rat heart lysates probed with Anti ADRB2 Polyclonal Antibody, Unconjugated (bs-0947R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 46kD. Observed band size: 46kD
Mouse splenoctyes probed with Rabbit Anti-ADRB2 Polyclonal Antibody (bs-0947R) at 1:100 for 30 minutes followed by incubation with Anti-Rabbit IgG FITC conjugated secondary compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ADRB2 Polyclonal Antibody, Unconjugated (bs-0947R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Stomach tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Rat Heart tissue lysates; Lane 5: Rat Stomach tissue lysates; Lane 6: Rat Cerebrum tissue lysates; Lane 7: Human A431 cell lysates probed with ADRB2 Polyclonal Antibody, Unconjugated (bs-0947R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.