DATASHEET
Host:
Rabbit
Target Protein:
ADRB2
Immunogen Range:
201-300/418
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
154
Swiss Prot:
P07550
Source:
KLH conjugated synthetic peptide derived from human of human ADRB2
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 3-fold greater affinity than it does norepinephrine.
PRODUCT SPECIFIC PUBLICATIONS
- Tibenska V et al. Gradual cold acclimation induces cardioprotection without affecting beta-adrenergic receptor-mediated adenylyl cyclase signaling. J Appl Physiol (1985). 2020 Apr 1;128(4):1023-1032.Read more>>
- Deng, Guo-Hua, et al. "Exogenous norepinephrine attenuates the efficacy of sunitinib in a mouse cancer model." Journal of Experimental & Clinical Cancer Research 33:21 (2014).Read more>>
- Revathikumar et al. Microsomal prostaglandin E synthase-1 gene deletion impairs neuro-immune circuitry of the cholinergic anti-inflammatory pathway in endotoxaemic mouse spleen. (2018) PLoS.One. 13:e0193210Read more>>
- Martín-Cordero L et al. β2 Adrenergic Regulation of the Phagocytic and Microbicide Capacity of Macrophages from Obese and Lean Mice: Effects of Exercise. Nutrients. 2019 Nov 9;11(11). pii: E2721. Read more>>
- Tibenska V et al. Gradual cold acclimation induces cardioprotection without affecting beta-adrenergic receptor-mediated adenylyl cyclase signaling. J Appl Physiol (1985). 2020 Apr 1;128(4):1023-1032.Read more>>
- Liu Z et al. Over-expression of microRNA-145 drives alterations in -adrenergic signaling and attenuates cardiac remodeling in heart failure post myocardial infarction. Aging (Albany NY). 2020 Jun 18;12(12):11603-11622.Read more>>
- Jinyao Liuet al. Alcohol consumption combined with dietary low-carbohydrate/high-protein intake increased the left ventricular systolic dysfunction risk and lethal ventricular arrhythmia susceptibility in apolipoprotein E/low-density lipoprotein receptor double-knockout mice. Alcohol. 2020 Dec;89:63-74.Read more>>
- Katarina Andelova. et al. Cardiac Cx43 Signaling Is Enhanced and TGF-β1/SMAD2/3 Suppressed in Response to Cold Acclimation and Modulated by Thyroid Status in Hairless SHRM. BIOMEDICINES. 2022 Jul;10(7):1707Read more>>
- Chuanju Zong. et al. Chronic restraint stress promotes gastric epithelial malignant transformation by activating the Akt/p53 signaling pathway via ADRB2. ONCOL LETT. 2022 Sep;24(3):1-11Read more>>
- Katsuaki Ieguchi. et al. The Sympathetic Nervous System Contributes to the Establishment of Pre-Metastatic Pulmonary Microenvironments. INT J MOL SCI. 2022 Jan;23(18):10652Read more>>
- Shuang Zhou. et al. Tumor microenvironment adrenergic nerves blockade liposomes for cancer therapy. J CONTROL RELEASE. 2022 Nov;351:656Read more>>
- Yunjung Lee. et al. Synergistic effect of n-3 PUFA and probiotic supplementation on bone loss induced by chronic mild stress through the brainCgutCbone axis. J FUNCT FOODS. 2023 Jan;100:105363Read more>>
- Lina S. Farhoumand. et al. Blockade of ß-Adrenergic Receptors by Nebivolol Enables Tumor Control Potential for Uveal Melanoma in 3D Tumor Spheroids and 2D Cultures. INT J MOL SCI. 2023 Jan;24(6):5894Read more>>
- Noam Ben-Shalom. et al. 2-Adrenergic Signaling Promotes Higher-Affinity B Cells and Antibodies. BRAIN BEHAV IMMUN. 2023 JuRead more>>
VALIDATION IMAGES
L1 rat brain lysates L2 rat heart lysates probed with Anti ADRB2 Polyclonal Antibody, Unconjugated (bs-0947R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 46kD. Observed band size: 46kD
Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Stomach tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Rat Heart tissue lysates; Lane 5: Rat Stomach tissue lysates; Lane 6: Rat Cerebrum tissue lysates; Lane 7: Human A431 cell lysates probed with ADRB2 Polyclonal Antibody, Unconjugated (bs-0947R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with ADRB2 polyclonal antibody, unconjugated (bs-0947R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.