Host: Rabbit
Target Protein: Endothelin-1
Immunogen Range: 53-73/202
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1906
Source: KLH conjugated synthetic peptide derived from human human Endothelin-1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 2.5
Cross Reactive Species:
Human
Mouse
Rat
Pig
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Horse
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Hu, Zhibin, et al. "Comparative analysis of preservation method and intermittent perfusion volume on the expression of endothelial and inflammatory markers by coronal artery and myocardium in porcine donor hearts." ASAIO journal (American Society for Artificial Internal Organs: 1992) (2014).Read more>>
- Prayitnaningsih et al. Neuropathy optic glaucomatosa induced by systemic hypertension through activation endothelin-1 signaling pathway in central retinal artery in rats. (2016) Int.J.Ophthalmol. 9:1568-1577Read more>>
- DeNiro and Al-Mohanna Nuclear factor kappa-B signaling is integral to ocular neovascularization in ischemia-independent microenvironment. (2014) PLoS.One. 9:e101602Read more>>
- Tian B et al. Electrochemical Immunoassay of Endothelin-1 Based on a Fenton-Type Reaction Using Cu(II)-Containing Nanocomposites as NanozymesAnal Chem.2020 Dec 15;92(24):15916-15926.Read more>>
- Yanhong Wang. et al. Intermedin Alleviates Renal Ischemia-Reperfusion Injury and Enhances Neovascularization in Wistar Rats. Drug Des Dev Ther. 2020; 14: 4825C4834Read more>>
- Jinbo Li. et al. Low dose shikonin and anthracyclines coloaded liposomes induce robust immunogenetic cell death for synergistic chemo-immunotherapy. J Control Release. 2021 Jul;335:306Read more>>
- Jinbo Li. et al. Autophagy inhibition recovers deficient ICD-based cancer immunotherapy. BIOMATERIALS. 2022 Aug;287:121651Read more>>
- Yang Liu. et al. Platycodon grandiflorus polysaccharides deeply participate in the anti-chronic bronchitis effects of platycodon grandiflorus decoction, a representative of the lung and intestine are related. FRONT PHARMACOL. 2022 Sep 7;13:927384Read more>>
- Nataliia Hula. et al. The Long-Term Effects of Prenatal Hypoxia on Coronary Artery Function of the Male and Female Offspring. BIOMEDICINES. 2022 Dec;10(12):3019Read more>>
- Mohammed Shadi A. D.. et al. Antihypertensive, antioxidant, and renal protective impact of integrated GJD with captopril in spontaneously hypertensive rats. SCI REP-UK. 2023 Jul;13(1):1-14Read more>>
"Formalin-fixed and paraffin embedded Guinea pig skin tissue labeled with Anti-ET-1 Polyclonal Antibody, Unconjugated (bs-0954R) at 1:200, followed by conjugation to the secondary antibody and DAB staining\n"
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Endothelin 1 Polyclonal Antibody, Unconjugated (bs-0954R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Endothelin 1 Polyclonal Antibody, Unconjugated (bs-0954R) at 1:200 overnight at 4°C, DAB staining.