bs-0964R-FITC [Conjugated Primary Antibody]
TRIM32/BBS11 Polyclonal Antibody, FITC Conjugated
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: TRIM32/BBS11

Immunogen Range: 301-400/655


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 22954

Source: KLH conjugated synthetic peptide derived from mouse TRIM32

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Tripartite motif-containing protein 32 (TRIM32) belongs to the tripartite motif (TRIM) protein family. TRIM32, like all TRIM proteins, contains a domain structure composed of a B-box, a RING-finger and a coiled-coil motif. Additionally, TRIM32 has six C-terminal NHL domains; it is expressed mainly in the skeletal muscle. The TRIM32 gene encodes an E3 ubiquitin ligase, a protein that attaches ubiquitin to a lysine residue on a target protein and acts in conjunction with ubiquitin-conjugating enzymes UbcH5a, UbcH5c and UbcH6. Mutations in the TRIM32 gene cause two forms of autosomal recessive muscular dystrophy designated limb girdle muscular dystrophy type 2H (LGMD2H) and sarcotubular myopathy (STM). TRIM32 mutations can also result in Bardet-Biedl syndrome (BBS), an autosomal recessive disorder characterized by pigmentary retinopathy, polydactyly, hypogenitalism, renal abnormalities, learning disabilities and obesity.

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 72


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lixiang Gu. et al. Mercuric chloride induced brain toxicity in mice: The protective effects of puerarin-loaded PLGA nanoparticles. J BIOCHEM MOL TOXIC. 2023 Jul;:e23425Read more>>
  • Fan Jin. et al. Quercetin alleviates kidney damage caused by mercury Chloride: The protective effects of quercetin on autophagy and inflammation were studied based on TRIM32/TLR4/LC3 pathway. TOXICON. 2024 Sep;248:108031Read more>>
VALIDATION IMAGES