bs-0996R [Primary Antibody]
Eph receptor B2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: EphB2

Immunogen Range: 551-650/1055


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2048

Swiss Prot: P29323

Source: KLH conjugated synthetic peptide derived from human Eph receptor B2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 108


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Choi, Won Hoon, et al. "Open-gate mutants of the mammalian proteasome show enhanced ubiquitin-conjugate degradation." Nature Communications 7 (2016).Read more>>
  • Yang Xuesong. et al. Intervention Mechanism of Hunag-Lian Jie-Du Decoction on Canonical Wnt/_-Catenin Signaling Pathway in Psoriasis Mouse Model. EVID-BASED COMPL ALT. 2022;2022:3193572Read more>>
  • Junjun Ling. et al. EPHB2 as a recurrence-related gene and a prognostic indicator in nasopharyngeal carcinoma: A bioinformatics screening and immunohistochemistry verification. HISTOL HISTOPATHOL. 2022 Apr 20;18459Read more>>
VALIDATION IMAGES

"Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-EphB2/Eph receptor B2 Polyclonal Antibody, Unconjugated (bs-0996R) at 1:200, followed by conjugation to the secondary antibody and DAB staining\n"


A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Eph receptor B2 Polyclonal Antibody(bs-0996R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Lane 1: Mouse Testis lysates; Lane 2: Rat Testis lysates; Lane 3: Human HUVEC cell lysates; Lane 4: Human U251 cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human U-2OS cell lysates; Lane 7: Human MDA-MB-231 cell lysates; Lane 8: Human K562 cell lysates probed with Eph receptor B2 Polyclonal Antibody, Unconjugated (bs-0996R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.