bs-10006R [Primary Antibody]
CTLA4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CTLA4

Immunogen Range: 75-170/223


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1493

Swiss Prot: P16410

Source: KLH conjugated synthetic peptide derived from human CTLA-4/CD152

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 21


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Seike, Masahiro, et al. "Histamine suppresses regulatory T cells mediated by TGF‐β in murine chronic allergic contact dermatitis." Experimental Dermatology(2015).Read more>>
  • Schlößer et al. Immune checkpoints programmed death 1 ligand 1 and cytotoxic T lymphocyte associated molecule 4 in gastric adenocarcinoma. (2016) Oncoimmunolog. 5:e1100789Read more>>
  • Jiang Minxiao. et al. Identification of a Hypoxia-Related Signature for Predicting Prognosis and the Immune Microenvironment in Bladder Cancer. Front Mol Biosci. 2021 May;8:38Read more>>
  • Li Lanzhou. et al. Calf Thymus Polypeptide Restrains the Growth of Colorectal Tumor via Regulating the Intestinal Microbiota-Mediated Immune Function. FRONT PHARMACOL. 2022 May;0:1645Read more>>
  • Weiqi Meng. et al. ZhenQi FuZheng formula inhibits the growth of colorectal tumors by modulating intestinal microflora-mediated immune function. AGING-US. 2022 Jun 15; 14(11): 47694785Read more>>
VALIDATION IMAGES

Mouse lung lysate probed with Anti CTLA4 Polyclonal Antibody, Unconjugated (bs-10006R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 34kD. Observed band size: 34kD