bs-10021R
[Primary Antibody]
NLRP3 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CIAS1
Immunogen Range: 1-50/1036
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 114548
Swiss Prot: Q96P20
Source: KLH conjugated synthetic peptide derived from human NLRP3
Purification: Purified by Protein A.
Storage: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Background: May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zhou, Tianwei E., et al. "Choroidal Involution Is Associated with a Progressive Degeneration of the Outer Retinal Function in a Model of Retinopathy of Prematurity: Early Role for IL-1β." The American Journal of Pathology (2016).Read more>>
- Fernandez, Melissa Victoria, et al. "Ion efflux and influenza infection trigger NLRP3 inflammasome signaling in human dendritic cells." Journal of leukocyte biology 99.5 (2016): 723-734.Read more>>
- Debye, Berthold, et al. "Neurodegeneration and NLRP3 inflammasome expression in the anterior thalamus of SOD1 (G93A) ALS mice." Brain Pathology (2016).Read more>>
- Heitzer, Marius, et al. "Administration of 17β-Estradiol Improves Motoneuron Survival and Down-regulates Inflammasome Activation in Male SOD1 (G93A) ALS Mice." Molecular Neurobiology: 1-15.Read more>>
- Zendedel, Adib, et al. "Estrogen Attenuates Local Inflammasome Expression and Activation after Spinal Cord Injury." Molecular Neurobiology (2017): 1-12.Read more>>
- Yu, Chengyuan, et al. "Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway." Oncotarget 8.23 (2017): 37705.Read more>>
- Yu, Chengyuan, et al. "Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway." Oncotarget 8.23 (2017): 37705.Read more>>
- Zhang, Xiaohui, et al. "H3 Relaxin Protects Against Myocardial Injury in Experimental Diabetic Cardiomyopathy by Inhibiting Myocardial Apoptosis, Fibrosis and Inflammation." Cellular Physiology and Biochemistry 43.4 (2017): 1311-1324.Read more>>
- Debye et al. Neurodegeneration and NLRP3 inflammasome expression in the anterior thalamus of SOD1(G93A) ALS mice. (2016) Brain.Pathol. Read more>>
- Qu X et al. Autophagy inhibition‐enhanced assembly of the NLRP3 inflammasome is associated with cisplatin‐induced acute injury to the liver and kidneys in rats. (2018) J Biochem Mol Toxicol.e22208.Read more>>
- Niu X et al. Harmine mitigates LPS-induced acute kidney injury through inhibition of the TLR4-NF-κB/NLRP3 inflammasome signalling pathway in mice. Eur J Pharmacol. 2019 Apr 15;849:160-169. Read more>>
- Peng XP et al. The protective effect of oleanolic acid on NMDA-induced MLE-12 cells apoptosis and lung injury in mice by activating SIRT1 and reducing NF-κB acetylation.Int Immunopharmacol. 2019 May;70:520-529.Read more>>
- Qu XY et al. Astragaloside IV protects against cisplatin-induced liver and kidney injury via autophagy-mediated inhibition of NLRP3 in rats.The Journal of Toxicological Sciences,2019 44(3), 167–175.Read more>>
- Kariya S et al. NLRP3 inflammasome expression in lipopolysaccharide-induced otitis media. Acta Otolaryngol. 2018 Dec;138(12):1061-1065. Read more>>
- Qu XY et al. XingNaoJing injections protect against cerebral ischemia/reperfusion injury and alleviate blood-brain barrier disruption in rats, through an underlying mechanism of NLRP3 inflammasomes suppression. Chin J Nat Med. 2019 Jul;17(7):498-505.Read more>>
- Chen Y et al. Curcumin attenuates potassium oxonate-induced hyperuricemia and kidney inflammation in mice. Biomed Pharmacother. 2019 Jul 27;118:109195.Read more>>
- Zhou Y et al. Exposure of DBP in Gestation Induces Inflammation of Testicular Sertoli Cells in Progeny by Activating NLRP3 Inflammasomes. Sci Total Environ. 2020 Mar 10;707:136139. Read more>>
- Yang X et al. HJ22, a Novel derivative of piperine, Attenuates ibotenic acid-induced cognitive impairment, oxidativestress, apoptosis and inflammation via inhibiting the protein-protein interaction of Keap1-Nrf2. Int Immunopharmacol. 2020 Mar 16;83:106383.Read more>>
- Guan X et al. Estrogen deficiency aggravates apical periodontitis by regulating NLRP3/caspase-1/IL-1β axis. Am J Transl Res. 2020 Feb 15;12(2):660-671.Read more>>
- Zhou Y et al. Exposure of DBP in Gestation Induces Inflammation of Testicular Sertoli Cells in Progeny by Activating NLRP3 Inflammasomes. Sci Total Environ. 2020 Mar 10;707:136139. Read more>>
- Zhou Y et al. Exposure of DBP in Gestation Induces Inflammation of Testicular Sertoli Cells in Progeny by Activating NLRP3 Inflammasomes. Sci Total Environ. 2020 Mar 10;707:136139. Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat kidney labeled with Anti-Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
This image was generously provided by Adib Zendedel, PhD from RWTH Aachen University. Formalin-fixed and paraffin embedded rat spinal cord tissue labeled with Rabbit Anti-Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:300 followed by conjugation to a secondary antibody
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti Cryopyrin/CIAS1/NALP3 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti Cryopyrin/CIAS1/NALP3 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining