VALIDATION IMAGES
Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat kidney labeled with Anti-Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
This image was generously provided by Adib Zendedel, PhD from RWTH Aachen University. Formalin-fixed and paraffin embedded rat spinal cord tissue labeled with Rabbit Anti-Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:300 followed by conjugation to a secondary antibody
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti Cryopyrin/CIAS1/NALP3 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti Cryopyrin/CIAS1/NALP3 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Raji lysates; Lane 2: MDA-MB-231 probed with Cryopyrin Polyclonal Antibody (bs-10021R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
SiHa lysates probed with Rabbit Anti-Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse Spleen lysates; Lane 2: Mouse Thymus lysates probed with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lymph node lysates probed with Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1:Rat Thymus lysates probed with NLRP3 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse RAW264.7 cell lysates; Lane 2: Mouse Thymus lysates probed with Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
