bs-10021R [Primary Antibody]
NALP3/CIAS1 Polyclonal Antibody
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DATASHEET

Host: Rabbit

Target Protein: NALP3/CIAS1

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 114548

Swiss Prot: Q96P20

Source: KLH conjugated synthetic peptide derived from human Cryopyrin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Size: 100µL

Concentration: 1mg/ml

Predicted Molecular Weight: 114


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhou, Tianwei E., et al. "Choroidal Involution Is Associated with a Progressive Degeneration of the Outer Retinal Function in a Model of Retinopathy of Prematurity: Early Role for IL-1β." The American Journal of Pathology (2016).Read more>>
  • Fernandez, Melissa Victoria, et al. "Ion efflux and influenza infection trigger NLRP3 inflammasome signaling in human dendritic cells." Journal of leukocyte biology 99.5 (2016): 723-734.Read more>>
  • Debye, Berthold, et al. "Neurodegeneration and NLRP3 inflammasome expression in the anterior thalamus of SOD1 (G93A) ALS mice." Brain Pathology (2016).Read more>>
  • Heitzer, Marius, et al. "Administration of 17β-Estradiol Improves Motoneuron Survival and Down-regulates Inflammasome Activation in Male SOD1 (G93A) ALS Mice." Molecular Neurobiology: 1-15.Read more>>
  • Zendedel, Adib, et al. "Estrogen Attenuates Local Inflammasome Expression and Activation after Spinal Cord Injury." Molecular Neurobiology (2017): 1-12.Read more>>
  • Yu, Chengyuan, et al. "Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway." Oncotarget 8.23 (2017): 37705.Read more>>
  • Yu, Chengyuan, et al. "Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway." Oncotarget 8.23 (2017): 37705.Read more>>
  • Zhang, Xiaohui, et al. "H3 Relaxin Protects Against Myocardial Injury in Experimental Diabetic Cardiomyopathy by Inhibiting Myocardial Apoptosis, Fibrosis and Inflammation." Cellular Physiology and Biochemistry 43.4 (2017): 1311-1324.Read more>>
  • Debye et al. Neurodegeneration and NLRP3 inflammasome expression in the anterior thalamus of SOD1(G93A) ALS mice. (2016) Brain.Pathol. Read more>>
  • Qu X et al. Autophagy inhibition‐enhanced assembly of the NLRP3 inflammasome is associated with cisplatin‐induced acute injury to the liver and kidneys in rats. (2018) J Biochem Mol Toxicol.e22208.Read more>>
  • Niu X et al. Harmine mitigates LPS-induced acute kidney injury through inhibition of the TLR4-NF-κB/NLRP3 inflammasome signalling pathway in mice. Eur J Pharmacol. 2019 Apr 15;849:160-169. Read more>>
  • Peng XP et al. The protective effect of oleanolic acid on NMDA-induced MLE-12 cells apoptosis and lung injury in mice by activating SIRT1 and reducing NF-κB acetylation.Int Immunopharmacol. 2019 May;70:520-529.Read more>>
  • Qu XY et al. Astragaloside IV protects against cisplatin-induced liver and kidney injury via autophagy-mediated inhibition of NLRP3 in rats.The Journal of Toxicological Sciences,2019 44(3), 167–175.Read more>>
  • Kariya S et al. NLRP3 inflammasome expression in lipopolysaccharide-induced otitis media. Acta Otolaryngol. 2018 Dec;138(12):1061-1065. Read more>>
  • Qu XY et al. XingNaoJing injections protect against cerebral ischemia/reperfusion injury and alleviate blood-brain barrier disruption in rats, through an underlying mechanism of NLRP3 inflammasomes suppression. Chin J Nat Med. 2019 Jul;17(7):498-505.Read more>>
  • Chen Y et al. Curcumin attenuates potassium oxonate-induced hyperuricemia and kidney inflammation in mice. Biomed Pharmacother. 2019 Jul 27;118:109195.Read more>>
  • Zhou Y et al. Exposure of DBP in Gestation Induces Inflammation of Testicular Sertoli Cells in Progeny by Activating NLRP3 Inflammasomes. Sci Total Environ. 2020 Mar 10;707:136139. Read more>>
  • Yang X et al. HJ22, a Novel derivative of piperine, Attenuates ibotenic acid-induced cognitive impairment, oxidativestress, apoptosis and inflammation via inhibiting the protein-protein interaction of Keap1-Nrf2. Int Immunopharmacol. 2020 Mar 16;83:106383.Read more>>
  • Guan X et al. Estrogen deficiency aggravates apical periodontitis by regulating NLRP3/caspase-1/IL-1β axis. Am J Transl Res. 2020 Feb 15;12(2):660-671.Read more>>
  • Zhou Y et al. Exposure of DBP in Gestation Induces Inflammation of Testicular Sertoli Cells in Progeny by Activating NLRP3 Inflammasomes. Sci Total Environ. 2020 Mar 10;707:136139. Read more>>
  • Zhou Y et al. Exposure of DBP in Gestation Induces Inflammation of Testicular Sertoli Cells in Progeny by Activating NLRP3 Inflammasomes. Sci Total Environ. 2020 Mar 10;707:136139. Read more>>
  • Hanwen Li et al. P2Y 14 receptor has a critical role in acute gouty arthritis by regulating pyroptosis of macrophages. Cell Death Dis.2020 May 26;11(5):394.Read more>>
  • Guan L et al. Puerarin ameliorates retinal ganglion cell damage induced by retinal ischemia/reperfusion through inhibiting the activation of TLR4/NLRP3 inflammasome. Life Sci. 2020 Sep 1;256:117935.Read more>>
  • Chen Y et al. A selected small molecule prevents inflammatory osteolysis through restraining osteoclastogenesis by modulating PTEN activityClin Transl Med.2020 Dec;10(8):e240.Read more>>
  • Liu P et al. Harmine Ameliorates Cognitive Impairment by Inhibiting NLRP3 Inflammasome Activation and Enhancing the BDNF/TrkB Signaling Pathway in STZ-Induced Diabetic Rats. Front Pharmacol. 2020 May 1;11:535.Read more>>
  • Zhu Hui-Zheng. et al. Xiaoyaosan Exerts Therapeutic Effects on the Colon of Chronic Restraint Stress Model Rats via the Regulation of Immunoinflammatory Activation Induced by the TLR4/NLRP3 Inflammasome Signaling Pathway. Evid-Based Compl Alt. 2021;2021:6673538Read more>>
  • Dong Zhixia. et al. Adiponectin Inhibits NLRP3 Inflammasome Activation in Nonalcoholic Steatohepatitis via AMPK-JNK/ErK1/2-NFB/ROS Signaling Pathways. Front Med-Lausanne. 2020 Nov;7:705Read more>>
  • Hui Che. et al. Melatonin exerts neuroprotective effects by inhibiting neuronal pyroptosis and autophagy in STZ\induced diabetic mice. Faseb J. 2020 Oct;34(10):14042-14054Read more>>
  • Lei Xianghong. et al. Micheliolide Attenuates Lipopolysaccharide-Induced Inflammation by Modulating the mROS/NF-B/NLRP3 Axis in Renal Tubular Epithelial Cells. Mediat Inflamm. 2020;2020:3934769Read more>>
  • Wei Yu. et al. Curcumin suppresses doxorubicin-induced cardiomyocyte pyroptosis via a PI3K/Akt/mTOR-dependent manner. Cardiovasc Diagn The. 2020 Aug; 10(4): 752C769Read more>>
  • Carmen Hummel. et al. Expression and Cell Type-specific Localization of Inflammasome Sensors in the Spinal Cord of SOD1(G93A) Mice and Sporadic Amyotrophic lateral sclerosis Patients. Neuroscience. 2021 MaRead more>>
  • Dai Jiangwen. et al. Liver kinase B1 attenuates liver ischemia/reperfusion injury via inhibiting the NLRP3 inflammasome. Acta Bioch Bioph Sin. 2021 MaRead more>>
  • Xiping Yang. et al. The piperine derivative HJ105 inhibits A1-42-induced neuroinflammation and oxidative damage via the Keap1-Nrf2-TXNIP axis. Phytomedicine. 2021 Apr;:153571Read more>>
  • Yaru Huang. et al. Dexmedetomidine attenuates myocardial ischemia-reperfusion injury in vitro by inhibiting NLRP3 Inflammasome activation. Bmc Anesthesiol. 2021 Dec;21(1):1-12Read more>>
  • Huan Xiao. et al. Effects of Mycobacterium vaccae Aerosol Inhalation on Airway Inflammation in Asthma Mouse Model. 2021 May 04Read more>>
  • You Zhou. et al. Overexpression of lncRNA TUG1 Alleviates NLRP3 Inflammasome-Mediated Cardiomyocyte Pyroptosis Through Targeting the miR-186-5p/XIAP Axis in Coronary Microembolization-Induced Myocardial Damage. Front Immunol. 2021; 12: 637598Read more>>
  • Chinmaya Panda. et al. Aggregated Tau-PHF6 (VQIVYK) Potentiates NLRP3 Inflammasome Expression and Autophagy in Human Microglial Cells. Cells-Basel. 2021 Jul;10(7):1652Read more>>
  • Zheng, Jie. et al. A novel function of NLRP3 independent of inflammasome as a key transcription factor of IL-33 in epithelial cells of atopic dermatitis. Cell Death Dis. 2021 Sep;12(10):1-12Read more>>
  • Wen Lianghe. et al. Melatonin Exerts Cardioprotective Effects by Inhibiting NLRP3 Inflammasome-Induced Pyroptosis in Mice following Myocardial Infarction. Oxid Med Cell Longev. 2021;2021:5387799Read more>>
  • Maryam Baazm. et al. Regulation of Inflammasomes by Application of Omega-3 Polyunsaturated Fatty Acids in a Spinal Cord Injury Model. Cells-Basel. 2021 Nov;10(11):3147Read more>>
  • Jie Ma. et al. The Effect of MaxiK Channel on Regulating the Activation of NLRP3 Inflammasome in Rats of Blast-induced Traumatic Brain Injury. Neuroscience. 2022 Feb;482:132Read more>>
  • Zijie Wei. et al. KDM2B overexpression prevents myocardial ischemia?reperfusion injury in rats through regulating inflammatory response via the TLR4/NF?B p65 axis. Exp Ther Med. 2022 Feb;23(2):1-8Read more>>
  • Shi-Yong Zhu. et al. Lycopene Ameliorates Atrazine-Induced Pyroptosis in Spleen via Suppressing the Ox-mtDNA/Nlrp3 Inflammasome Pathway. Food Funct. 2021 NovRead more>>
  • Meiyun Cai. et al. Kaemperfol alleviates pyroptosis and microglia-mediated neuroinflammation in Parkinson's disease via inhibiting p38MAPK/NF-B signaling pathway. Neurochem Int. 2021 Nov;:105221Read more>>
  • Shi, Chenxi. et al. Dihydromyricetin alleviates Escherichia coli lipopolysaccharide-induced hepatic injury in chickens by inhibiting the NLRP3 inflammasome. Vet Res. 2022 Dec;53(1):1-16Read more>>
  • Pang, Qi. et al. Irisin protects against vascular calcification by activating autophagy and inhibiting NLRP3-mediated vascular smooth muscle cell pyroptosis in chronic kidney disease. Cell Death Dis. 2022 Mar;13(3):1-15Read more>>
  • Meng Sun. et al. Silibinin protects against sepsis and septic myocardial injury in an NR1H3-dependent pathway. FREE RADICAL BIO MED. 2022 Jul;187:141Read more>>
  • Jian Kang. et al. Ginsenoside Rg1 Mitigates Porcine Intestinal Tight Junction Disruptions Induced by LPS through the p38 MAPK/NLRP3 Inflammasome Pathway. TOXICS. 2022 Jun;10(6):285Read more>>
  • Ming-Hui Li. et al. Fufang Zhenzhu Tiaozhi capsule ameliorates hyperuricemic nephropathy by inhibition of PI3K/AKT/NF-B pathway. J ETHNOPHARMACOL. 2022 Aug;:115644Read more>>
  • Li, Xiao. et al. Synergistic neuroprotective effect of saikosaponin A and albiflorin on corticosterone-induced apoptosis in PC12 cells via regulation of metabolic disorders and neuroinflammation. MOL BIOL REP. 2022 Aug;:1-13Read more>>
VALIDATION IMAGES

This image was generously provided by Adib Zendedel, PhD from RWTH Aachen University. Formalin-fixed and paraffin embedded rat spinal cord tissue labeled with Rabbit Anti-Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:300 followed by conjugation to a secondary antibody


Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Cryopyrin Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated (bs-10021R-A488) 1:400, 90 minutes at 37°C; DAPI (5ug/ml, blue) was used to stain the cell nuclei.


Lane 1: Raji lysates; Lane 2: MDA-MB-231 probed with Cryopyrin Polyclonal Antibody (bs-10021R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.


SiHa lysates probed with Rabbit Anti-Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.


Lane 1: Mouse Spleen lysates; Lane 2: Mouse Thymus lysates probed with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Lymph node lysates probed with Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Lane 1:Rat Thymus lysates probed with NLRP3 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Lane 1: Mouse RAW264.7 cell lysates; Lane 2: Mouse Thymus lysates probed with Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C


Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Large intestine lysates; Lane 3: Mouse Thymus cell lysates; Lane 4: Mouse Lung cell lysates; Lane 5: Rat Cerebrum lysates; Lane 6: Rat Thymus lysates; Lane 7: Rat Lung lysates; Lane 8: Human Lung cell lysates probed withCryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


RAW264.7 (M) cells were treated with or without LPS (10 µg/ml) for 8h, 25 μg total protein per lane of cell lysates (see on figure) probed with NALP3/CIAS1 polyclonal antibody, unconjugated (bs-10021R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Purulent appendicitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Chronic inflammatory mastitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (bs-10021R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with NALP3/CIAS1 polyclonal antibody, unconjugated (bs-10021R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.