bs-10027R [Primary Antibody]
NCR1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NCR1

Immunogen Range: 198-206/304


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9437

Swiss Prot: O76036

Source: KLH conjugated synthetic peptide derived from human CD335

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)

Predicted Molecular Weight: 31


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • VINDEVOGEL, EVA, et al. "The Use of Toll-like Receptor 4 Agonist to Reshape the Immune Signature in Ovarian Cancer." Anticancer Research36.11 (2016): 5781-5792.Read more>>
  • Jaime-Ramirez, Alena C., et al. "Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma." Molecular Therapy-Oncolytics 5 (2017): 87-96.Read more>>
  • Pan, Pan, et al. "Black Raspberries Enhance Natural Killer Cell Infiltration into the Colon and Suppress the Progression of Colorectal Cancer." Frontiers in Immunology 8 (2017): 997.Read more>>
  • Jaime-Ramirez et al. Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma. (2017) Mol.Ther.Oncolytics. 5:87-96Read more>>
  • Pan et al. Black Raspberries Enhance Natural Killer Cell Infiltration into the Colon and Suppress the Progression of Colorectal Cancer. (2017) Front.Immunol. 8:997Read more>>
VALIDATION IMAGES

Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Spleen lysates probed with NCR1 Polyclonal Antibody, Unconjugated (bs-10027R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Kidney lysates; Lane 2: Mouse Spleen lysates; Lane 3: Mouse Lymph node lysates; Lane 4: A431 cell lysates ; Lane 5: Raji cell lysates probed with NCR1 Polyclonal Antibody, Unconjugated (bs-10027R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Spleen lysates; Lane 3: Mouse Lung lysates probed with NCR1 Polyclonal Antibody, Unconjugated (bs-10027R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Rat Lymph node tissue lysates; Lane 4: Human HL-60 cell lysates probed with NCR1 Polyclonal Antibody, Unconjugated (bs-10027R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.