bs-10032R [Primary Antibody]
beta casein Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: beta casein

Immunogen Range: 16-100/224


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 281099

Swiss Prot: P02666

Source: KLH conjugated synthetic peptide derived from bovine Beta-casein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Important role in determination of the surface properties of the casein micelles. Casoparan acts as a macrophage activator, increasing the phagocytic activity of macrophages and peroxide release from macrophages. It also acts as a bradykinin-potentiating peptide. Casohypotensin acts as a bradykinin-potentiating peptide. Induces hypotension in rats. Acts as a strong competitive inhibitor of endo-oligopeptidase A. Antioxidant peptide has antioxidant activity.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-F(1:100-500)

Predicted Molecular Weight: 24


Cross Reactive Species: Human
Bovine
Goat
Cow

Predicted Cross Reactive Species: Sheep

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Petrášová, Michaela, et al. "Immunofluorescence detection of milk protein in meat products." Potravinarstvo 9.1 (2015): 101-105.Read more>>
  • Wang B et al. Laminin-dependent integrin β1 signaling regulates milk protein synthesis via prolactin/STAT5 pathway in bovine mammary epithelial cells. Biochem Biophys Res Commun. 2020 Jan 24. Read more>>
  • Hou et al. Spleen tyrosine kinase regulates mammary epithelial cell proliferation in mammary glands of dairy cows. (2016) J.Dairy.Sci. 99:3858-68Read more>>
  • Wang B et al. Laminin-dependent integrin β1 signaling regulates milk protein synthesis via prolactin/STAT5 pathway in bovine mammary epithelial cells. Biochem Biophys Res Commun. 2020 Jan 24. Read more>>
  • Chaochao Luo. et al. Glutamine Regulates Cell Growth and Casein Synthesis through the CYTHs/ARFGAP1-Arf1-mTORC1 Pathway in Bovine Mammary Epithelial Cells. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXXRead more>>
  • Zong, Jinxin. et al. Lithium Chloride Promotes Milk Protein and Fat Synthesis in Bovine Mammary Epithelial Cells via HIF-1_ and _-Catenin Signaling Pathways. Biol Trace Elem Res. 2022 Jan;:1-16Read more>>
  • Sanam Foroutanparsa. et al. Spatial distribution of s1-caseins and -caseins in milk gels acidified with glucono--lactone. FOOD HYDROCOLLOID. 2023 Jan;:108506Read more>>
  • Minghui Zhang. et al. Comparative Transcriptomic Analysis of Mammary Gland Tissues Reveals the Critical Role of GPR110 in Palmitic Acid-Stimulated Milk Protein and Fat Synthesis. BRIT J NUTR. 2023 Mar;:1-32Read more>>
  • Xiaoyu Duan. et al. mTORC2-AKT-LAT1 Signalling Participates in Methionine-induced -CASEIN Expression in Mammary Epithelial Cells of Dairy Cows. J ANIM PHYSIOL AN N. 2023 MaRead more>>
VALIDATION IMAGES

Lane 1: Milk (Goat) Lysate at 2 ug Lane 2: Milk (Cow) Lysate at 30 ug Lane 3: Milk (Cow) Lysate at 3 ug Lane 4: Milk (Human) Lysate at 30 ug Primary: Anti-Beta-casein (bs-10032R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution


Cow milk probed with Rabbit Anti-Beta-casein Polyclonal Antibody, Unconjugated (bs-10032R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.