bs-10034R [Primary Antibody]
Alpha s2 Casein Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Alpha s2 Casein

Immunogen Range: 165-203/222


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 282209

Swiss Prot: P02663

Source: KLH conjugated synthetic peptide derived from cow Alpha s2 Casein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Important role in the capacity of milk to transport calcium phosphate. Casocidin-I inhibits the growth of E.coli and S.carnosus.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Predicted Molecular Weight: 24


Cross Reactive Species: Mouse
Bovine

Predicted Cross Reactive Species: Cow
Sheep

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gao, H. N., et al. "Leucine and histidine independently regulate milk protein synthesis in bovine mammary epithelial cells via mTOR signaling pathway." J. Zhejiang Univ.-Sci. B (Biomed. & Biotechnol.) 16.6 (2015): 560-572.Read more>>
  • Luo et al. SESN2 negatively regulates cell proliferation and casein synthesis by inhibition the amino acid-mediated mTORC1 pathway in cow mammary epithelial cells. (2018) Sci.Rep. 8:3912Read more>>
  • Chiara Rossi. et al. Reaction of cysteine residues with oxidized tyrosine residues mediates cross-linking of photo-oxidized casein proteins. Food Chem. 2022 Aug;385:132667Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded bovine breast tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-alpha S2 casein Polyclonal Antibody, Unconjugated (bs-10034R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining