bs-1003R [Primary Antibody]
EAAT1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: EAAT1

Immunogen Range: 301-400/512

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6507

Swiss Prot: P43003

Source: KLH conjugated synthetic peptide derived from human EAAT1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 60

Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xiao, Ke-Qing, et al. "Effect of subarachnoid nerve block anesthesia on glutamate transporter GLAST and GLT-1 expressions in rabbits." Asian Pacific Journal of Tropical Medicine (2015).Read more>>
  • Lu, Jun, et al. "Expression of GFAP, GLAST and GLT-1 in astrocytes in patients with mesial temporal lobe epilepsy." Journal of Hainan Medical University 22.2 (2016): 87-90.Read more>>
  • Ohmichi T et al.Quantification of brain-derived extracellular vesicles in plasma as a biomarker to diagnose Parkinson's and related diseases.(2018)Parkinsonism Relat Disord. Nov 20.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-EAAT1/GLAST Polyclonal Antibody (bs-1003R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining


Lane 1: rat heart lysates Lane 2: rat brain lysates probed with Anti EAAT1/Glast Polyclonal Antibody, Unconjugated (bs-1003R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 56kD. Observed band size: 56kD