bs-10055R-A594 [Conjugated Primary Antibody]
RIPK1 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: RIPK1

Immunogen Range: 151-250/671


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8737

Swiss Prot: Q13546

Source: KLH conjugated synthetic peptide derived from human RIPK1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.

Conjugation: ALEXA FLUOR® 594

Excitation/ Emission: 590nm/617nm

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 74


Cross Reactive Species: Human

Predicted Cross Reactive Species: Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Deutsch, M., et al. "Divergent effects of RIP1 or RIP3 blockade in murine models of acute liver injury." Cell death & disease 6.5 (2015): e1759.Read more>>
  • You et al. Effects of silencing RIP1 with siRNA on the biological behavior of the LoVo human colon cancer cell line. (2014) Oncol.Let. 7:2065-2072Read more>>
  • Derya Karabulut. et al. Effects of vitamin B12 on methotrexate hepatotoxicity: evaluation of receptor-interacting protein (RIP) kinase. N-S Arch Pharmacol. 2020 Dec;393(12):2473-248Read more>>
VALIDATION IMAGES