bs-10076R [Primary Antibody]
ROR1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ROR1

Immunogen Range: 301-360/937


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4919

Swiss Prot: Q01973

Source: KLH conjugated synthetic peptide derived from human ROR1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Tyrosine-protein kinase receptor whose role is not yet clear. ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 100


Cross Reactive Species: Human

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Antigen: bs-10067P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\nTMB staining;\nRead the data in MicroplateReader by 450


Lane 1:A549 cell lysates; Lane 2: 293T cell lysates; Lane 3: MDA-MB-231 cell lysates probed with ROR1 Polyclonal Antibody, Unconjugated (bs-10076R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.