DATASHEET
Host:
Rabbit
Target Protein:
Tau protein Ser552
Modification Site:
Ser552
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
4137
Source:
KLH conjugated synthetic phosphopeptide derived from human Tau protein around the phosphorylation site of Ser552
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Tau proteins are important Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. Tau proteins subcellular located in the axons of neurons, in the cytoso l and in association with plasma membrane components. It expressed in neurons. PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
VALIDATION IMAGES
Lane 1: Mouse hippocampus lysates;Lane 2:Mouse Cerebellum lysates; Lane 3: Mouse cerebrum lysates probed with Tau protein(Ser552) Polyclonal Antibody, Unconjugated (bs-10111R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebellum lysates; Lane 2: Rat Cerebrum lysates probed with Tau protein(Ser552) Polyclonal Antibody, Unconjugated (bs-10111R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.